Open Access

7,8‑Dihydroxyflavone activates Nrf2/HO‑1 signaling pathways and protects against osteoarthritis

  • Authors:
    • Dawei Cai
    • Wan Feng
    • Jun Liu
    • Longhai Jiang
    • Sichun Chen
    • Tangbo Yuan
    • Chen Yu
    • Hao Xie
    • Dawei Geng
    • Jian Qin
  • View Affiliations

  • Published online on: July 8, 2019     https://doi.org/10.3892/etm.2019.7745
  • Pages: 1677-1684
  • Copyright: © Cai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate the effect of 7,8‑dihydroxyflavone (7,8‑DHF) against osteoarthritis (OA) and examine its regulatory role in the nuclear factor (erythroid‑derived 2)‑like 2 (Nrf2) signaling pathway in chondrocytes. Primary mouse chondrocytes were treated with 7,8‑DHF to examine the expression of Nrf2 and downstream heme oxygenase 1 (HO‑1). The surgical destabilization of the medial meniscus model was used to assess the effectiveness of 7,8‑DHF in protecting the cartilage from damage, with knee cartilage harvested from mice for histological analysis. The results revealed that 7,8‑DHF activated the Nrf2 signaling pathway in primary chondrocytes. Cartilage degradation in the 7,8‑DHF‑treated group was reduced significantly compared with that in the vehicle‑treated group, according to histological evaluation. The gene expression of matrix metalloproteinase (MMP)1, MMP3, MMP13, interleukin (IL)‑1β, IL‑6 and tumor necrosis factor‑α were reduced in the cartilage of OA mice following 7,8‑DHF treatment. Genetic and protein analyses indicated that the expression levels of HO‑1 were upregulated in the cartilage of the knee with OA, and 7,8‑DHF treatment further promoted the induction of HO‑1. These results suggest that 7,8‑DHF may serve as a potential therapeutic agent in OA.

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September 2019
Volume 18 Issue 3

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Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Cai, D., Feng, W., Liu, J., Jiang, L., Chen, S., Yuan, T. ... Qin, J. (2019). 7,8‑Dihydroxyflavone activates Nrf2/HO‑1 signaling pathways and protects against osteoarthritis. Experimental and Therapeutic Medicine, 18, 1677-1684. https://doi.org/10.3892/etm.2019.7745
MLA
Cai, D., Feng, W., Liu, J., Jiang, L., Chen, S., Yuan, T., Yu, C., Xie, H., Geng, D., Qin, J."7,8‑Dihydroxyflavone activates Nrf2/HO‑1 signaling pathways and protects against osteoarthritis". Experimental and Therapeutic Medicine 18.3 (2019): 1677-1684.
Chicago
Cai, D., Feng, W., Liu, J., Jiang, L., Chen, S., Yuan, T., Yu, C., Xie, H., Geng, D., Qin, J."7,8‑Dihydroxyflavone activates Nrf2/HO‑1 signaling pathways and protects against osteoarthritis". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1677-1684. https://doi.org/10.3892/etm.2019.7745