Downregulation of miR‑3934‑5p enhances A549 cell sensitivity to cisplatin by targeting TP53INP1
- Aijun Ren
- Zhenzhen Wen
- Liangjie Zheng
Published online on: July 1, 2019
Copyright: © Ren et al.
This is an open access article distributed under the terms of Creative Commons Attribution License.
| PMC Statistics:
Total PDF Downloads:
| PMC Statistics:
Tumor protein p53‑inducible nuclear protein 1 (TP53INP1) is a tumor suppressor associated with malignant tumor metastasis. In addition, it has been reported that hsa‑microRNA (miR)‑3934 serves key roles in various types of lung cancer, including small‑cell lung carcinomas (SCLC) and non‑SCLC (NSCLC). Therefore, the present study aimed to determine the effects of miR‑3934‑5p on cell proliferation and apoptosis, and on sensitivity to cisplatin (DDP). Reverse transcription‑quantitative polymerase chain reaction analysis and western blotting were conducted for the analysis of mRNA and protein expression, respectively. Furthermore, the target of miR‑3934‑5p was investigated using a luciferase reporter assay and apoptosis was analyzed by flow cytometry. The results demonstrated that miR‑3934‑5p was upregulated in NSCLC tissues and A549 cells. Increases in the half‑maximal inhibitory concentration (IC50) and the expression of miR‑3934‑5p were observed in the A549/DDP group. miR‑3934‑5p mimic promoted the expression of miR‑3934‑5p and the IC50 of the A549 cells. miR‑3934‑5p inhibitor downregulated miR‑3934‑5p and reduced the IC50 of A549/DDP cells. miR‑3934‑5p was revealed to target the 3'‑untranslated region of TP53INP1. The downregulation of miR‑3934‑5p significantly suppressed the proliferation and promoted the apoptosis of A549/DDP cells, which were reversed by transfection with TP53INP1 small interfering (si)RNA. The protein and mRNA expression levels of TP53INP1, B‑cell lymphoma 2 (Bcl‑2)‑associated‑X and p21 were significantly increased, whereas those of Bcl‑2 were significantly decreased in the miR‑3934‑5p inhibitor group, which was significantly reduced by TP53INP1 siRNA transfection. miR‑3934‑5p, as a tumor suppressor in NSCLC, may promote the sensitivity of cells to DDP by targeting TP53INP1, associated with the suppression of cell proliferation and promotion of apoptosis.