Open Access

Mechanical loading mediates human nucleus pulposus cell viability and extracellular matrix metabolism by activating of NF‑κB

  • Authors:
    • Kai Zhang
    • Chao Xue
    • Ning Lu
    • Peng Ren
    • Haiwen Peng
    • Yao Wang
    • Yan Wang
  • View Affiliations

  • Published online on: July 8, 2019     https://doi.org/10.3892/etm.2019.7744
  • Pages: 1587-1594
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Lower back pain is one of the most frequent complaints in US orthopedic outpatient departments. Intervertebral disc degeneration (IDD) is an important cause of lower back pain. Previous studies have found that mechanical loading was associated with IDD, but the underlying mechanism remains unclear. In the present study, a human nucleus pulposus cell line was used to establish an in vitro mechanical loading model. Mechanical loading, western blot analysis, quantitative PCR, ELISA, cell viability assay and IHC staining were used in the current study. It was found that a short loading time of 4 h followed by a long period of rest (20 h) exerted protective effects against matrix degradation in nucleus pulposus cells, whilst a longer loading time of 20 h followed by a shorter period of rest (4 h) resulted in cell apoptosis and extracellular matrix (ECM) degradation. Excessive mechanical loading may induce ECM degradation by activation of the NF‑κB signaling pathway. Taken together, these findings demonstrated that whilst moderate mechanical loading exerted beneficial effects on nucleus pulposus cells, excessive mechanical loading inhibited human nucleus pulposus cell viability and promoted ECM degradation by activating NF‑κB.

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September 2019
Volume 18 Issue 3

Print ISSN: 1792-0981
Online ISSN:1792-1015

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Copy and paste a formatted citation
APA
Zhang, K., Xue, C., Lu, N., Ren, P., Peng, H., Wang, Y., & Wang, Y. (2019). Mechanical loading mediates human nucleus pulposus cell viability and extracellular matrix metabolism by activating of NF‑κB. Experimental and Therapeutic Medicine, 18, 1587-1594. https://doi.org/10.3892/etm.2019.7744
MLA
Zhang, K., Xue, C., Lu, N., Ren, P., Peng, H., Wang, Y., Wang, Y."Mechanical loading mediates human nucleus pulposus cell viability and extracellular matrix metabolism by activating of NF‑κB". Experimental and Therapeutic Medicine 18.3 (2019): 1587-1594.
Chicago
Zhang, K., Xue, C., Lu, N., Ren, P., Peng, H., Wang, Y., Wang, Y."Mechanical loading mediates human nucleus pulposus cell viability and extracellular matrix metabolism by activating of NF‑κB". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1587-1594. https://doi.org/10.3892/etm.2019.7744