Open Access

Probiotic effect on Helicobacter pylori attachment and inhibition of inflammation in human gastric epithelial cells

  • Authors:
    • Hanyi Song
    • Long Zhou
    • Dongyan Liu
    • Lihui Ge
    • Yan Li
  • View Affiliations

  • Published online on: July 5, 2019     https://doi.org/10.3892/etm.2019.7742
  • Pages: 1551-1562
  • Copyright: © Song et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Helicobacter pylori (H. pylori) is a major cause of chronic gastritis, gastric ulcers and gastric cancer. Recent studies have identified that probiotics are beneficial to human health due, in part, to their anti‑H. pylori activities. Therefore, the present study investigated the antagonistic and local immunoregulatory activities of seven commercial probiotic strains and explored their mechanisms of actions. The human gastric epithelial cell line‑1 (GES‑1) was used to assess the effects of probiotics on the adhesion ability of H. pylori. GES‑1 cells were infected with H. pylori plus lipopolysaccharide (HP‑LPS) or the drug‑resistant H. pylori strain (HP021) in the presence or absence of live probiotics. Following this, the growth rate and the adhesion ability of GES‑1 cells were detected using MTT and urease activity assay. Toll‑like receptor 4 (TLR4), NFKB inhibitor‑α (IκBα) and nuclear factor (NF)‑κB levels were measured by western blot analysis. The amount of interleukin (IL)‑8 in the cell culture medium was determined by ELISA. Amongst the seven probiotic strains studied, live Lactobacillus acidophilus (L. acidophilus) and Lactobacillus bulgaricus (L. bulgaricus) inhibited H. pylori adherence to GES‑1 cells most significantly. L. bulgaricus inhibited IL‑8 production by GES‑1 cells through modulation of the TLR4/IκBα/NF‑κB pathway. Therefore, the present results suggested that consumption of food containing L. acidophilus and L. bulgaricus may be used as an adjuvant therapy for H. pylori‑associated gastritis.

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September 2019
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Copy and paste a formatted citation
APA
Song, H., Zhou, L., Liu, D., Ge, L., & Li, Y. (2019). Probiotic effect on Helicobacter pylori attachment and inhibition of inflammation in human gastric epithelial cells. Experimental and Therapeutic Medicine, 18, 1551-1562. https://doi.org/10.3892/etm.2019.7742
MLA
Song, H., Zhou, L., Liu, D., Ge, L., Li, Y."Probiotic effect on Helicobacter pylori attachment and inhibition of inflammation in human gastric epithelial cells". Experimental and Therapeutic Medicine 18.3 (2019): 1551-1562.
Chicago
Song, H., Zhou, L., Liu, D., Ge, L., Li, Y."Probiotic effect on Helicobacter pylori attachment and inhibition of inflammation in human gastric epithelial cells". Experimental and Therapeutic Medicine 18, no. 3 (2019): 1551-1562. https://doi.org/10.3892/etm.2019.7742