Expression and function of miR‑155 in rat synovial fibroblast model of rheumatoid arthritis

  • Authors:
    • Hewei Li
    • Ping Liu
    • Yanlin Gong
    • Jiali Liu
    • Feng Ruan
  • View Affiliations

  • Published online on: May 15, 2019     https://doi.org/10.3892/etm.2019.7581
  • Pages: 786-792
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Rheumatoid arthritis (RA) is a common autoimmune disease characterized by joint synovial inflammation and is a challenge for researchers and clinicians. MicroRNAs (miRNAs/miRs) represent a group of small non‑coding RNA molecules that post‑transcriptionally regulate mRNA expression and are involved in various diseases, including cancer, autoimmune and metabolic diseases, as well as neurological disorders. In the present study, various experiments were performed to investigate the effects and underlying mechanism of miR‑155 in RA using rat synoviocytes induced by lipopolysaccharide (LPS) to model rheumatoid arthritis. It was revealed that synovial fibroblasts exhibited significantly higher miR‑155 mRNA levels than the control group. Compared with the RA group, the viability of synovial fibroblasts was significantly decreased in the miR‑155 mimics + RA group, but markedly increased in the miR‑155 inhibitor + RA group. Compared with that in the RA + NC mimic or RA + NC inhibitor groups, the apoptosis of synovial fibroblasts increased significantly in the miR‑155 mimics + RA group, but was significantly decreased in the miR‑155 inhibitor + RA group. The miR‑155 mimics + RA group exhibited higher expression levels of β‑catenin, matrix metalloproteinase 7 and cyclin D1 compared with the miR‑155 inhibitor + RA group, and the glycogen synthase kinase protein levels was lower compared with the miR‑155 inhibitor + RA group. In brief, it was inferred that the Wnt signaling pathway is involved in the miR‑155‑associated inhibition of RA synovial fibroblast viability and induction of cell apoptosis. Inhibition of miR‑155 may be an effective treatment for RA through regulation of the Wnt signaling pathway to reduce cell apoptosis and enhance cell viability.

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July 2019
Volume 18 Issue 1

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APA
Li, H., Liu, P., Gong, Y., Liu, J., & Ruan, F. (2019). Expression and function of miR‑155 in rat synovial fibroblast model of rheumatoid arthritis. Experimental and Therapeutic Medicine, 18, 786-792. https://doi.org/10.3892/etm.2019.7581
MLA
Li, H., Liu, P., Gong, Y., Liu, J., Ruan, F."Expression and function of miR‑155 in rat synovial fibroblast model of rheumatoid arthritis". Experimental and Therapeutic Medicine 18.1 (2019): 786-792.
Chicago
Li, H., Liu, P., Gong, Y., Liu, J., Ruan, F."Expression and function of miR‑155 in rat synovial fibroblast model of rheumatoid arthritis". Experimental and Therapeutic Medicine 18, no. 1 (2019): 786-792. https://doi.org/10.3892/etm.2019.7581