Long non‑coding RNA BLACAT1 promotes the proliferation and invasion of glioma cells via Wnt/β‑catenin signaling

  • Authors:
    • Xiushan Li
    • Shujing Qi
    • Dongzhou Ma
    • Jinbiao Fan
    • Jingtao Wang
  • View Affiliations

  • Published online on: April 5, 2019     https://doi.org/10.3892/etm.2019.7468
  • Pages: 4703-4708
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Abstract

Long non‑coding RNAs (lncRNAs) are hypothesized to regulate numerous biological behaviors in human cancers. The present study aimed to explore the roles of lncRNA bladder cancer associated transcript 1 (BLACAT1) in glioma. The expression of BLACAT1 in glioma tissues and cell lines was determined by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). CCK‑8 assay, colony formation assay, wound healing assay and Transwell invasion assay were used to explore the roles of BLACAT1 in glioma cells. RT‑qPCR and western blot analysis were used to determine the BLACAT1 molecular mechanism. The findings demonstrated that lncRNA BLACAT1 was overexpressed in glioma tissues and cell lines. High BLACAT1 expression was correlated with high tumor grade in glioma patients. Functional assays determined that BLACAT1 promoted glioma cell proliferation, migration, invasion and epithelial‑mesenchymal transition in vitro. In addition, it was demonstrated that BLACAT1 activated the Wnt/β‑catenin signaling pathway. In conclusion, BLACAT1 may serve as an oncogenic lncRNA in glioma progression via activation of the Wnt/β‑catenin signaling pathway. Therefore, BLACAT1 may be a novel therapeutic target for glioma treatment.

References

1 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2016. CA Cancer J Clin. 66:7–30. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Schwartzbaum JA, Fisher JL, Aldape KD and Wrensch M: Epidemiology and molecular pathology of glioma. Nat Clin Pract Neurol. 2:494–503. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Trabelsi S, Brahim DH, Ladib M, Mama N, Harrabi I, Tlili K, Yacoubi MT, Krifa H, Hmissa S, Saad A and Mokni M: Glioma epidemiology in the central tunisian population: 1993–2012. Asian Pac J Cancer Prev. 15:8753–8757. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Wrensch M, Fisher JL, Schwartzbaum JA, Bondy M, Berger M and Aldape KD: The molecular epidemiology of gliomas in adults. Neurosurg Focus. 19:E52005. View Article : Google Scholar : PubMed/NCBI

5 

Reich DE, Cargill M, Bolk S, Ireland J, Sabeti PC, Richter DJ, Lavery T, Kouyoumjian R, Farhadian SF, Ward R and Lander ES: Linkage disequilibrium in the human genome. Nature. 411:199–204. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Lander ES: Initial impact of the sequencing of the human genome. Nature. 470:187–197. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Mercer TR, Dinger ME and Mattick JS: Long non-coding RNAs: Insights into functions. Nat Rev Genet. 10:155–159. 2009. View Article : Google Scholar : PubMed/NCBI

8 

Shi X, Sun M, Liu H, Yao Y and Song Y: Long non-coding RNAs: A new frontier in the study of human diseases. Cancer Lett. 339:159–166. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Monnier P, Martinet C, Pontis J, Stancheva I, Ait-Si-Ali S and Dandolo L: H19 lncRNA controls gene expression of the Imprinted Gene Network by recruiting MBD1. Proc Natl Acad Sci U S A. 110:20693–20698. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Lee JT: Epigenetic regulation by long noncoding RNAs. Science. 338:1435–1439. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Zhou M, Wang X, Shi H, Cheng L, Wang Z, Zhao H, Yang L and Sun J: Characterization of long non-coding RNA-associated ceRNA network to reveal potential prognostic lncRNA biomarkers in human ovarian cancer. Oncotarget. 7:12598–12611. 2016.PubMed/NCBI

12 

Feng W, Li L, Xu X, Jiao Y and Du W: Up-regulation of the long non-coding RNA RMRP contributes to glioma progression and promotes glioma cell proliferation and invasion. Arch Med Sci. 13:1315–1321. 2017. View Article : Google Scholar : PubMed/NCBI

13 

Wang XP, Shan C, Deng XL, Li LY and Ma W: Long non-coding RNA PAR5 inhibits the proliferation and progression of glioma through interaction with EZH2. Oncol Rep. 38:3177–3186. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Ma Y, Zhou G, Li M, Hu D, Zhang L, Liu P and Lin K: Long noncoding RNA DANCR mediates cisplatin resistance in glioma cells via activating AXL/PI3K/Akt/NF-κB signaling pathway. Neurochem Int. 118:233–241. 2018. View Article : Google Scholar : PubMed/NCBI

15 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

16 

Alonso MM, Diez-Valle R, Manterola L, Rubio A, Liu D, Cortes-Santiago N, Urquiza L, Jauregi P, Lopez de Munain A, Sampron N, et al: Genetic and epigenetic modifications of Sox2 contribute to the invasive phenotype of malignant gliomas. PLoS One. 6:e267402011. View Article : Google Scholar : PubMed/NCBI

17 

Chen X, Dai M, Zhu H, Li J, Huang Z, Liu X, Huang Y, Chen J and Dai S: Evaluation on the diagnostic and prognostic values of long non-coding RNA BLACAT1 in common types of human cancer. Mol Cancer. 16:1602017. View Article : Google Scholar : PubMed/NCBI

18 

Shan D, Shang Y and Hu T: Long noncoding RNA BLACAT1 promotes cell proliferation and invasion in human cervical cancer. Oncol Lett. 15:3490–3495. 2018.PubMed/NCBI

19 

Ye JR, Liu L and Zheng F: Long noncoding RNA bladder cancer associated transcript 1 promotes the proliferation, migration, and invasion of nonsmall cell lung cancer through sponging miR-144. DNA Cell Biol. 36:845–852. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Wu X, Zheng Y, Han B and Dong X: Long noncoding RNA BLACAT1 modulates ABCB1 to promote oxaliplatin resistance of gastric cancer via sponging miR-361. Biomed Pharmacother. 99:832–838. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Liao D, Lv G, Wang T, Min J, Wang Y and Liu S: Prognostic value of long non-coding RNA BLACAT1 in patients with papillary thyroid carcinoma. Cancer Cell Int. 18:472018. View Article : Google Scholar : PubMed/NCBI

22 

Yang J and Weinberg RA: Epithelial-mesenchymal transition: At the crossroads of development and tumor metastasis. Dev Cell. 14:818–829. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Li Y, Huang S, Li Y, Zhang W, He K, Zhao M, Lin H, Li D, Zhang H, Zheng Z and Huang C: Decreased expression of LncRNA SLC25A25-AS1 promotes proliferation, chemoresistance, and EMT in colorectal cancer cells. Tumor Biol. 37:14205–14215. 2016. View Article : Google Scholar

24 

He R, Zhang FH and Shen N: LncRNA FEZF1-AS1 enhances epithelial-mesenchymal transition (EMT) through suppressing E-cadherin and regulating WNT pathway in non-small cell lung cancer (NSCLC). Biomed Pharmacother. 95:331–338. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Nusse R and Clevers H: Wnt/β-catenin signaling, disease, and emerging therapeutic modalities. Cell. 169:985–999. 2017. View Article : Google Scholar : PubMed/NCBI

26 

Tian L, Zhao Z, Xie L and Zhu J: MiR-361-5p inhibits the mobility of gastric cancer cells through suppressing epithelial-mesenchymal transition via the Wnt/β-catenin pathway. Gene. 675:102–109. 2018. View Article : Google Scholar : PubMed/NCBI

27 

Yuan H, Wei R, Xiao Y, Song Y, Wang J, Yu H, Fang T, Xu W and Mao S: RHBDF1 regulates APC-mediated stimulation of the epithelial-to-mesenchymal transition and proliferation of colorectal cancer cells in part via the Wnt/β-catenin signalling pathway. Exp Cell Res. 368:24–36. 2018. View Article : Google Scholar : PubMed/NCBI

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June 2019
Volume 17 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Li, X., Qi, S., Ma, D., Fan, J., & Wang, J. (2019). Long non‑coding RNA BLACAT1 promotes the proliferation and invasion of glioma cells via Wnt/β‑catenin signaling. Experimental and Therapeutic Medicine, 17, 4703-4708. https://doi.org/10.3892/etm.2019.7468
MLA
Li, X., Qi, S., Ma, D., Fan, J., Wang, J."Long non‑coding RNA BLACAT1 promotes the proliferation and invasion of glioma cells via Wnt/β‑catenin signaling". Experimental and Therapeutic Medicine 17.6 (2019): 4703-4708.
Chicago
Li, X., Qi, S., Ma, D., Fan, J., Wang, J."Long non‑coding RNA BLACAT1 promotes the proliferation and invasion of glioma cells via Wnt/β‑catenin signaling". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4703-4708. https://doi.org/10.3892/etm.2019.7468