Open Access

Abnormal expression and mutation of the RBPJ gene may be involved in CD59‑ clonal proliferation in paroxysmal nocturnal hemoglobinuria

  • Authors:
    • Liyan Li
    • Hui Liu
    • Honglei Wang
    • Zhaoyun Liu
    • Yingying Chen
    • Lijuan Li
    • Jia Song
    • Guojin Wang
    • Rong Fu
  • View Affiliations

  • Published online on: April 10, 2019     https://doi.org/10.3892/etm.2019.7475
  • Pages: 4536-4546
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Paroxysmal nocturnal hemoglobinuria (PNH) is an acquired clonal proliferative disease of hematopoietic stem cells. Various gene mutations, including the phosphatidylinositol glycan anchor biosynthesis class A (PIG‑A) gene, may contribute to the proliferation of PNH clones. In order to explore the mechanism of PNH clone proliferation, a study was performed on 13 patients with PNH who underwent whole exome sequencing. The frequency of mutations in these patients was explored, and an additional 30 patients with PNH were selected for analysis of cluster of differentiation 59‑negative (CD59‑) cells. The mRNA expression of 13 genes, which were selected based on their high frequency in patients with PNH and the fact that they met four screening conditions, was determined in these CD59‑ cells. Cell proliferation, apoptosis and cell cycle were evaluated upon knocking down the recombinant signal binding protein of immunoglobulin κJ region (RBPJ) gene in 5 patients in vitro. The detection rate of PIG‑A gene mutation was 61.54% (8/13), and additional mutations in somatic genes were detected, including RBPJ, zinc finger protein 717, polycomb repressive complex 2 subunit and tet methylcytosine dioxygenase. Upon screening according to the mutation frequency and expression level, the present study focused on the RBPJ gene. The expression level of RBPJ in CD59‑ cells was apparently higher than that in CD59+ cells and normal controls which was significantly correlated with clinical data. Furthermore, the expression of RBPJ in PNH primary cells could be effectively inhibited by small interfering RNA‑RBPJ. Once the expression of RBPJ decreased remarkably, the apoptotic rate increased gradually, while cell proliferation activity decreased with transfection time and cells were blocked in G0/G1 phase. In conclusion, mutations and abnormal expression of the RBPJ gene may participate in the abnormal proliferation of PNH clones.
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June 2019
Volume 17 Issue 6

Print ISSN: 1792-0981
Online ISSN:1792-1015

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APA
Li, L., Liu, H., Wang, H., Liu, Z., Chen, Y., Li, L. ... Fu, R. (2019). Abnormal expression and mutation of the RBPJ gene may be involved in CD59‑ clonal proliferation in paroxysmal nocturnal hemoglobinuria. Experimental and Therapeutic Medicine, 17, 4536-4546. https://doi.org/10.3892/etm.2019.7475
MLA
Li, L., Liu, H., Wang, H., Liu, Z., Chen, Y., Li, L., Song, J., Wang, G., Fu, R."Abnormal expression and mutation of the RBPJ gene may be involved in CD59‑ clonal proliferation in paroxysmal nocturnal hemoglobinuria". Experimental and Therapeutic Medicine 17.6 (2019): 4536-4546.
Chicago
Li, L., Liu, H., Wang, H., Liu, Z., Chen, Y., Li, L., Song, J., Wang, G., Fu, R."Abnormal expression and mutation of the RBPJ gene may be involved in CD59‑ clonal proliferation in paroxysmal nocturnal hemoglobinuria". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4536-4546. https://doi.org/10.3892/etm.2019.7475