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Investigation of expression and effects of TGF‑β1 and MMP‑9 in lens epithelial cells of diabetic cataract rats

  • Authors:
    • Ke Li
    • Wei Ai
  • View Affiliations

  • Published online on: April 8, 2019     https://doi.org/10.3892/etm.2019.7471
  • Pages: 4522-4526
  • Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expressions and effects of transforming growth factor‑ 1 (TGF‑β1) and matrix metalloproteinase‑9 (MMP‑9) in lens epithelial cells (LECs) of diabetic cataract rats were investigated. A total of 40 female Sprague‑Dawley rats were randomly divided into study and control group. Rats in study group were successfully modeled diabetic cataract rats, and rats in control group were normal rats. Immunohistochemical staining was used to determine positive and negative granules in cytoplasm, and image proplus image analysis system to calculate the integral optical density of the average positive area. Quantitative analysis was performed on TGF‑β1 and MMP‑9 in LECs of rats in study and control groups at the 2nd and 4th weekends. There were no statistically significant differences in length and age between the two groups of rats (P>0.05). Glucose concentration in the blood of rats in study group after modeling was significantly higher than that before modeling (P<0.001), and that after modeling was significantly higher in study group than that in control group (P<0.001). The expression of TGF‑β1 protein in LECs of rats in study group at T2 (the 4th weekend) was significantly higher than that at T1 (the 2nd weekend) (P<0.001), and that of TGF‑β1 protein was significantly higher in study group than that in control group at T1 and T2 (P<0.001). The expression of MMP‑9 protein in LECs of rats in study group at T2 was significantly higher than that at T1 (P<0.001), and that of MMP‑9 protein was significantly higher in study group than that in control group at T1 and T2 (P<0.001). The TGF‑β1 expression was positively correlated with the MMP‑9 expression in LECs of diabetic cataract rats (r=0.825, P<001). The increased expression of MMP‑9 and TGF‑β1 may play an important role in the occurrence and development of diabetic cataract.

References

1 

Chang KC, Li L, Sanborn TM, Shieh B, Lenhart P, Ammar D, LaBarbera DV and Petrash JM: Characterization of Emodin as a therapeutic agent for diabetic cataract. J Nat Prod. 79:1439–1444. 2016. View Article : Google Scholar : PubMed/NCBI

2 

Tan JS, Wang JJ and Mitchell P: Influence of diabetes and cardiovascular disease on the long-term incidence of cataract: The Blue Mountains eye study. Ophthalmic Epidemiol. 15:317–327. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Lu Q, Hao M, Wu W, Zhang N, Isaac AT, Yin J, Zhu X, Du L and Yin X: Antidiabetic cataract effects of GbE, rutin and quercetin are mediated by the inhibition of oxidative stress and polyol pathway. Acta Biochim Pol. 65:35–41. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Bhadada SV, Vyas VK and Goyal RK: Protective effect of Tephrosia purpurea in diabetic cataract through aldose reductase inhibitory activity. Biomed Pharmacother. 83:221–228. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Lee JY, Jeong HS, Lee DY, Sohn HJ and Nam DH: Early postoperative intraocular pressure stability after combined 23-gauge sutureless vitrectomy and cataract surgery in patients with proliferative diabetic retinopathy. Retina. 32:1767–1774. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Takamura Y, Sugimoto Y, Kubo E, Takahashi Y and Akagi Y: Immunohistochemical study of apoptosis of lens epithelial cells in human and diabetic rat cataracts. Jpn J Ophthalmol. 45:559–563. 2001. View Article : Google Scholar : PubMed/NCBI

7 

de Iongh RU, Wederell E, Lovicu FJ and McAvoy JW: Transforming growth factor-beta-induced epithelial-mesenchymal transition in the lens: A model for cataract formation. Cells Tissues Organs. 179:43–55. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Hales AM, Chamberlain CG, Murphy CR and McAvoy JW: Estrogen protects lenses against cataract induced by transforming growth factor-beta (TGFbeta). J Exp Med. 185:273–280. 1997. View Article : Google Scholar : PubMed/NCBI

9 

Xu GX, Hu JZ, Zheng WD, Zhang S and Wang TT: Expression and significance of transforming growth factor-beta1, matrix metalloproteinase-2 and its inhibitor in lens epithelial cells of diabetic cataract. Zhonghua Yan Ke Za Zhi. 39:411–414. 2003.(In Chinese). PubMed/NCBI

10 

Nathu Z, Dwivedi DJ, Reddan JR, Sheardown H, Margetts PJ and West-Mays JA: Temporal changes in MMP mRNA expression in the lens epithelium during anterior subcapsular cataract formation. Exp Eye Res. 88:323–330. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Kwon JW, Choi JA and Jee D: Matrix metalloproteinase-1 and matrix metalloproteinase-9 in the aqueous humor of diabetic macular edema patients. PLoS One. 11:e01597202016. View Article : Google Scholar : PubMed/NCBI

12 

Dwivedi DJ, Pino G, Banh A, Nathu Z, Howchin D, Margetts P, Sivak JG and West-Mays JA: Matrix metalloproteinase inhibitors suppress transforming growth factor-beta-induced subcapsular cataract formation. Am J Pathol. 168:69–79. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Boscia F, Grattagliano I, Vendemiale G, Micelli-Ferrari T and Altomare E: Protein oxidation and lens opacity in humans. Invest Ophthalmol Vis Sci. 41:2461–2465. 2000.PubMed/NCBI

14 

Lindsey ML, Iyer RP, Jung M, DeLeon-Pennell KY and Ma Y: Matrix metalloproteinases as input and output signals for post-myocardial infarction remodeling. J Mol Cell Cardiol. 91:134–140. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Backstrom JR and Tökés ZA: The 84-kDa form of human matrix metalloproteinase-9 degrades substance P and gelatin. J Neurochem. 64:1312–1318. 1995. View Article : Google Scholar : PubMed/NCBI

16 

Inoue Y, Abe K, Obata K, Yoshioka T, Ohmura G, Doh K, Yamamoto K, Hoshiai H and Noda K: Immunohistochemical studies on matrix metalloproteinase-9 (MMP-9) and type-IV collagen in endometrial carcinoma. J Obstet Gynaecol Res. 23:139–145. 1997. View Article : Google Scholar : PubMed/NCBI

17 

Luo D, Guan Q, Wang K, Nguan CYC and Du C: TGF-β1 stimulates movement of renal proximal tubular epithelial cells in a three-dimensional cell culture via an autocrine TGF-β2 production. Exp Cell Res. 350:132–139. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Kawarada Y, Inoue Y, Kawasaki F, Fukuura K, Sato K, Tanaka T, Itoh Y and Hayashi H: TGF-β induces p53/Smads complex formation in the PAI-1 promoter to activate transcription. Sci Rep. 6:354832016. View Article : Google Scholar : PubMed/NCBI

19 

Selim S: Frequency and pattern of chronic complications of diabetes and their association with glycemic control among adults with type 2 diabetes in Bangladesh. Diabetes Metab Syndr. 11 (Suppl 1):S329–S332. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Akiyode O and Tran C: Overview of ocular anti-vascular endothelial growth factor therapy in the management of diabetic eye complications. Diabetes Spectr. 29:44–49. 2016. View Article : Google Scholar : PubMed/NCBI

21 

Voelker J, Berg PH, Sheetz M, Duffin K, Shen T, Moser B, Greene T, Blumenthal SS, Rychlik I, Yagil Y, et al: Anti-TGF-β1 antibody therapy in patients with diabetic nephropathy. J Am Soc Nephrol. 28:953–962. 2017. View Article : Google Scholar : PubMed/NCBI

22 

Xu MT, Sun S, Zhang L, Xu F, Du SL, Zhang XD and Wang DW: Diabetes mellitus affects the biomechanical function of the callus and the expression of TGF-beta1 and BMP2 in an early stage of fracture healing. Braz J Med Biol Res. 49:e47362016.PubMed/NCBI

23 

Alapure BV, Praveen MR, Gajjar DU, Vasavada AR, Parmar TJ and Arora AI: Matrix metalloproteinase-2 and −9 activities in the human lens epithelial cells and serum of steroid induced posterior subcapsular cataracts. Mol Vis. 18:64–73. 2012.PubMed/NCBI

24 

Dynlacht JR: The role of age, sex and steroid sex hormones in radiation cataractogenesis. Radiat Res. 180:559–566. 2013. View Article : Google Scholar : PubMed/NCBI

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June 2019
Volume 17 Issue 6

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Copy and paste a formatted citation
APA
Li, K., & Li, K. (2019). Investigation of expression and effects of TGF‑β1 and MMP‑9 in lens epithelial cells of diabetic cataract rats. Experimental and Therapeutic Medicine, 17, 4522-4526. https://doi.org/10.3892/etm.2019.7471
MLA
Li, K., Ai, W."Investigation of expression and effects of TGF‑β1 and MMP‑9 in lens epithelial cells of diabetic cataract rats". Experimental and Therapeutic Medicine 17.6 (2019): 4522-4526.
Chicago
Li, K., Ai, W."Investigation of expression and effects of TGF‑β1 and MMP‑9 in lens epithelial cells of diabetic cataract rats". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4522-4526. https://doi.org/10.3892/etm.2019.7471