Open Access

Protective effect of gastrodin on myocardial ischemia‑reperfusion injury and the expression of Bax and Bcl‑2

  • Authors:
    • Xiao Han
    • Hai Shi
    • Kun Liu
    • Lou Zhong
    • Fei Wang
    • Qingsheng You
  • View Affiliations

  • Published online on: April 19, 2019     https://doi.org/10.3892/etm.2019.7512
  • Pages: 4389-4394
  • Copyright: © Han et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The protective effects of gastrodin on myocardial ischemia‑reperfusion injury in rats and the underlying mechanism were investigated. Sprague Dawley (SD) rats were randomly divided into three groups, of which the gastrodin group was treated with gastrodin, and the other two groups were treated with normal saline. In the myocardial ischemia-reperfusion model group, myocardial ischemia was induced by ligation of the left anterior descending coronary artery, and myocardial reperfusion was performed by ligature removal. Only thread without ligation for the sham operation group was conducted. The rats were euthanized 8 days after surgery. Heart tissues were harvested and used for measurement of apoptotic rate and expression levels of apoptosis‑related proteins. Serum levels of cytokines were measured also using blood samples. Compared with the myocardial ischemia‑reperfusion model group, significant reduction of cardiomyocyte apoptosis was observed in the gastrodin group (P<0.05). In the gastrodin group, the protein and mRNA expression levels for Bax and activated caspase‑3 decreased, while for Bcl‑2 increased (P<0.05). Gastrodin can downregulate inflammatory cytokines (P<0.05) and upregulate anti‑inflammatory cytokines such as IL‑10 (P<0.05) in serum of SD rats. Therefore, gastrodin played a protective role in myocardial ischemia‑reperfusion injury by regulating the expression levels of apoptosis‑related signaling proteins and inflammatory cytokines.

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Copy and paste a formatted citation
APA
Han, X., Shi, H., Liu, K., Zhong, L., Wang, F., & You, Q. (2019). Protective effect of gastrodin on myocardial ischemia‑reperfusion injury and the expression of Bax and Bcl‑2 . Experimental and Therapeutic Medicine, 17, 4389-4394. https://doi.org/10.3892/etm.2019.7512
MLA
Han, X., Shi, H., Liu, K., Zhong, L., Wang, F., You, Q."Protective effect of gastrodin on myocardial ischemia‑reperfusion injury and the expression of Bax and Bcl‑2 ". Experimental and Therapeutic Medicine 17.6 (2019): 4389-4394.
Chicago
Han, X., Shi, H., Liu, K., Zhong, L., Wang, F., You, Q."Protective effect of gastrodin on myocardial ischemia‑reperfusion injury and the expression of Bax and Bcl‑2 ". Experimental and Therapeutic Medicine 17, no. 6 (2019): 4389-4394. https://doi.org/10.3892/etm.2019.7512