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Testosterone modulation of ethanol effects on the µ‑opioid receptor kinetics in castrated rats

  • Authors:
    • Rafaat Khalil
    • Jessica Humann
  • View Affiliations

  • Published online on: July 18, 2019     https://doi.org/10.3892/br.2019.1230
  • Pages: 103-109
  • Copyright: © Khalil et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present investigation was conducted to evaluate the effects of testosterone on ethanol‑induced alterations of µ‑opioid receptor binding kinetics in specific brain regions of castrated rats. Male Sprague Dawley rats (100‑124 g) adapted to a 12‑h light/dark cycle were used. Animals were castrated under pentobarbital anesthesia. After a recovery period of 14 days, ethanol [3 g/kg as 22.5% solution in saline via intraperitoneal injection (i.p.)], testosterone [2.5 mg in 0.2 ml of olive oil via subcutaneous injection (s.c.) in the dorsal neck region] or the combination of ethanol and testosterone were administered to rats at 9:00 a.m. The control group was injected i.p. with 2 ml saline and s.c. with 0.2 ml olive oil for 7 days. Animals were sacrificed by decapitation at 2 h after the final injection. The brains were immediately removed, and the cortex, hippocampus, hypothalamus and midbrain were dissected. In an attempt to elucidate the mechanism involved in the hormonal modulation of the effects of ethanol and testosterone on the endogenous opioid system, the binding kinetics of the µ‑opioid receptors were determined. The results obtained in the present study assisted in identifying the regulatory role of testosterone on ethanol‑induced changes on µ‑opioid receptor binding kinetics.

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APA
Khalil, R., & Khalil, R. (2019). Testosterone modulation of ethanol effects on the µ‑opioid receptor kinetics in castrated rats. Biomedical Reports, 11, 103-109. https://doi.org/10.3892/br.2019.1230
MLA
Khalil, R., Humann, J."Testosterone modulation of ethanol effects on the µ‑opioid receptor kinetics in castrated rats". Biomedical Reports 11.3 (2019): 103-109.
Chicago
Khalil, R., Humann, J."Testosterone modulation of ethanol effects on the µ‑opioid receptor kinetics in castrated rats". Biomedical Reports 11, no. 3 (2019): 103-109. https://doi.org/10.3892/br.2019.1230