Open Access

Dexamethasone induces aberrant macrophage immune function and apoptosis

  • Authors:
    • Fulu Ai
    • Guohua Zhao
    • Wu Lv
    • Bin Liu
    • Jie Lin
  • View Affiliations

  • Published online on: December 13, 2019     https://doi.org/10.3892/or.2019.7434
  • Pages: 427-436
  • Copyright: © Ai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Glucocorticoids (GCs) are known potent clinical drugs, however, their mode of action is still complex and debatable. Macrophages are the most important target of GCs and play a key role in tumor immunity in vivo, but their relationship is also controversial. In the present study, the lentivirus system was used to overexpress and knock down the level of transcription factor Krüppel‑like factor 9 (KLF9). The results revealed that dexamethasone (Dex) induced ROS generation and mitochondria‑dependent apoptosis in RAW 264.7 cells via the KLF9. In addition, overexpression of KLF9 significantly increased apoptosis of RAW 264.7 cells. Notably, ELISA assay revealed that increased expression of KLF9 inhibited LPS‑induced COX‑2 expression and reduced COX‑2‑derived prostaglandin E2 and pro‑inflammatory cytokine secretion. Furthermore, a co‑culture system was used to reveal that overexpression of KLF9 in RAW 264.7 cells promoted HepG2 cell survival. In summary, it is reported that KLF9 promoted apoptosis of proinflammatory macrophages, and suppressed the antitumor effects, which can be selectively targeted by GCs as a novel mechanism to suppress antineoplastic activity.
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February 2020
Volume 43 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Ai, F., Zhao, G., Lv, W., Liu, B., & Lin, J. (2020). Dexamethasone induces aberrant macrophage immune function and apoptosis. Oncology Reports, 43, 427-436. https://doi.org/10.3892/or.2019.7434
MLA
Ai, F., Zhao, G., Lv, W., Liu, B., Lin, J."Dexamethasone induces aberrant macrophage immune function and apoptosis". Oncology Reports 43.2 (2020): 427-436.
Chicago
Ai, F., Zhao, G., Lv, W., Liu, B., Lin, J."Dexamethasone induces aberrant macrophage immune function and apoptosis". Oncology Reports 43, no. 2 (2020): 427-436. https://doi.org/10.3892/or.2019.7434