Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells

  • Authors:
    • Bin Dai
    • Yucheng Shen
    • Ting Yan
    • Ailiang Zhang
  • View Affiliations

  • Published online on: December 10, 2019     https://doi.org/10.3892/or.2019.7424
  • Pages: 601-608
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Receptor tyrosine kinase like orphan receptor 2 (ROR2) regulates Wnt5a-induced cell migration by phosphorylating PI3K/Akt and activating RhoA in osteosarcoma. However, the role of Wnt5a signaling and its corresponding receptors in the regulation of osteosarcoma metastasis remains poorly understood. ROR1 monoclonal antibody (mAb) and short hairpin (sh)RNA targeting ROR2 markedly inhibited the activity of dishevelled associated activator of morphogenesis 1 (DAAM1) and RhoA and retarded cell migration in osteosarcoma. ROR1 mAb and ROR2 shRNA destroyed the microfilament formation of osteosarcoma cells. Silencing of DAAM1 (with DAAM1 shRNA) downregulated RhoA activity and inhibited cell migration. The decrease of cell migration caused by DAAM1 shRNA was rescued by wild-type DAAM1 overexpression. DAAM1 and PI3Kα/Akt were parallel signaling pathways mediating osteosarcoma cell migration in response to Wnt5a. It was concluded that Wnt5a promotes osteosarcoma cell migration via ROR1/2 receptors, and then activates DAAM1 and RhoA.
View Figures
View References

Related Articles

Journal Cover

February 2020
Volume 43 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Dai, B., Shen, Y., Yan, T., & Zhang, A. (2020). Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells. Oncology Reports, 43, 601-608. https://doi.org/10.3892/or.2019.7424
MLA
Dai, B., Shen, Y., Yan, T., Zhang, A."Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells". Oncology Reports 43.2 (2020): 601-608.
Chicago
Dai, B., Shen, Y., Yan, T., Zhang, A."Wnt5a/ROR1 activates DAAM1 and promotes the migration in osteosarcoma cells". Oncology Reports 43, no. 2 (2020): 601-608. https://doi.org/10.3892/or.2019.7424