Open Access

Transcriptome analysis of dog oral melanoma and its oncogenic analogy with human melanoma

  • Authors:
    • Md. Mahfuzur Rahman
    • Yu‑Chang Lai
    • Al Asmaul Husna
    • Hui‑Wen Chen
    • Yuiko Tanaka
    • Hiroaki Kawaguchi
    • Hitoshi Hatai
    • Noriaki Miyoshi
    • Takayuki Nakagawa
    • Ryuji Fukushima
    • Naoki Miura
  • View Affiliations

  • Published online on: October 25, 2019     https://doi.org/10.3892/or.2019.7391
  • Pages: 16-30
  • Copyright: © Rahman et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Dogs have been considered as an excellent immunocompetent model for human melanoma due to the same tumor location and the common clinical and pathological features with human melanoma. However, the differences in the melanoma transcriptome between the two species have not been yet fully determined. Considering the role of oncogenes in melanoma development, in this study, we first characterized the transcriptome in canine oral melanoma and then compared the transcriptome with that of human melanoma. The global transcriptome from 8 canine oral melanoma samples and 3 healthy oral tissues were compared by RNA‑Seq followed by RT‑qPCR validation. The results revealed 2,555 annotated differentially expressed genes, as well as 364 novel differentially expressed genes. Dog chromosomes 1 and 9 were enriched with downregulated and upregulated genes, respectively. Along with 10 significant transcription site binding motifs; the NF‑κB and ATF1 binding motifs were the most significant and 4 significant unknown motifs were indentified among the upregulated differentially expressed genes. Moreover, it was found that canine oral melanoma shared >80% significant oncogenes (upregulated genes) with human melanoma, and JAK‑STAT was the most common significant pathway between the species. The results identified a 429 gene signature in melanoma, which was up‑regulated in both species; these genes may be good candidates for therapeutic development. Furthermore, this study demonstrates that as regards oncogene expression, human melanoma contains an oncogene group that bears similarities with dog oral melanoma, which supports the use of dogs as a model for the development of novel therapeutics and experimental trials before human application.
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January 2020
Volume 43 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Rahman, M., Lai, Y., Husna, A.A., Chen, H., Tanaka, Y., Kawaguchi, H. ... Miura, N. (2020). Transcriptome analysis of dog oral melanoma and its oncogenic analogy with human melanoma. Oncology Reports, 43, 16-30. https://doi.org/10.3892/or.2019.7391
MLA
Rahman, M., Lai, Y., Husna, A. A., Chen, H., Tanaka, Y., Kawaguchi, H., Hatai, H., Miyoshi, N., Nakagawa, T., Fukushima, R., Miura, N."Transcriptome analysis of dog oral melanoma and its oncogenic analogy with human melanoma". Oncology Reports 43.1 (2020): 16-30.
Chicago
Rahman, M., Lai, Y., Husna, A. A., Chen, H., Tanaka, Y., Kawaguchi, H., Hatai, H., Miyoshi, N., Nakagawa, T., Fukushima, R., Miura, N."Transcriptome analysis of dog oral melanoma and its oncogenic analogy with human melanoma". Oncology Reports 43, no. 1 (2020): 16-30. https://doi.org/10.3892/or.2019.7391