Open Access

Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells

  • Authors:
    • Xiaoyang Hou
    • Yanyu Pang
    • Xinxin Li
    • Chunsheng Yang
    • Wenlou Liu
    • Guan Jiang
    • Yanqun Liu
  • View Affiliations

  • Published online on: September 20, 2019     https://doi.org/10.3892/or.2019.7329
  • Copyright: © Hou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A novel core‑shell type thermo‑nanoparticle (CSTNP) co‑loaded with temozolomide (TMZ) and the fluorescein new indocyanine green dye IR820 (termed IT‑CSTNPs) was designed and combined with a near‑infrared (NIR) laser to realize its photothermal conversion. The IT‑CSTNPs were prepared using a two‑step synthesis method and comprised a thermosensitive shell and a biodegradable core. IR820 and TMZ were entrapped in the shell and the core, respectively. Dynamic light scattering results demonstrated that the average hydrodynamic size of the IT‑CSTNPs was 196.4±3.1 nm with a ζ potential of ‑24.9±1.3 mV. The encapsulation efficiencies of TMZ and IR820 were 6.1 and 16.6%, respectively. Temperature increase curves under NIR laser irradiation indicated that the IT‑CSTNPs exhibited the desired photothermal conversion efficiency. The in vitro drug release curves revealed a suitable release capability of IT‑CSTNP under physiological conditions, whereas NIR laser irradiation accelerated the drug release. Inverted fluorescence microscopy and flow cytometry results revealed that the uptake of IT‑CSTNPs by A375 melanoma cells occurred in a concentration‑dependent manner. Confocal laser scanning microscopy results indicated that IT‑CSTNPs entered tumour cells via endocytosis and were located in intercellular lysosomes. In summary, the present study explored the photothermal conversion capability, cellular uptake, and intracellular localization of IT‑CSTNPs.

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Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Hou, X., Pang, Y., Li, X., Yang, C., Liu, W., Jiang, G., & Liu, Y. (1899). Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells. Oncology Reports, 0, 0-0. https://doi.org/10.3892/or.2019.7329
MLA
Hou, X., Pang, Y., Li, X., Yang, C., Liu, W., Jiang, G., Liu, Y."Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells". Oncology Reports 0.0 (1899): 0-0.
Chicago
Hou, X., Pang, Y., Li, X., Yang, C., Liu, W., Jiang, G., Liu, Y."Core‑shell type thermo‑nanoparticles loaded with temozolomide combined with photothermal therapy in melanoma cells". Oncology Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/or.2019.7329