Anti-inflammatory effects of Calophyllum inophyllum L. in RAW264.7 cells
- Shih-Chang Tsai
- Yu-Hsin Liang
- Jo-Hua Chiang
- Fon-Chang Liu
- Wen-Hsin Lin
- Shu-Jen Chang
- Wei-Yu Lin
- Chieh-Hsi Wu
- Jing-Ru Weng
Affiliations: Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Life Sciences, National Chung Hsing University, Taichung 40227, Taiwan, R.O.C., School of Pharmacy, China Medical University, Taichung 40402, Taiwan, R.O.C., Department of Pharmacy, Kinmen Hospital, Kinmen 89142, Taiwan, R.O.C.
- Published online on: June 19, 2012 https://doi.org/10.3892/or.2012.1873
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Calophyllum inophyllum L. has been used as folk medicine in the treatment of ocular burn and it has demonstrated potential to be an anti-inflammatory agent. The aim of this study was to explore the anti-inflammatory activities of an acetone extract of C. inophyllum L. leaves (CIL). The CIL extract was tested on lipopolysaccharide (LPS)-induced RAW 264.7 cells to evaluate the effect of CIL extract on the expression of nitric oxide (NO) and inducible nitric oxide synthase (iNOS). Results showed that the CIL extract markedly suppressed the LPS-induced production of nitric oxide, as well as the expression of iNOS, cyclooxygenase (COX)-2 and nuclear factor-kappaB (NF-κB) in a dose-dependent manner. LPS-induced microRNA (miR)-146a expression was inhibited by CIL extract, while miR-155 and miR-424 expression was not affected as demonstrated using quantitative RT-PCR analysis. Taken together, these observations show that CIL extract has anti-inflammatory effect, which extends the potential application for prevention of inflammatory diseases, and its mechanism may be partially associated with blocking COX-2 and iNOS of RAW 264.7 cells.