Open Access

Involvement of ribosomal protein L11 expression in sensitivity of gastric cancer against 5‑FU

  • Authors:
    • Takuto Kawahata
    • Kohichi Kawahara
    • Michiko Shimokawa
    • Akie Sakiyama
    • Takehiro Shiraishi
    • Kentaro Minami
    • Masatatsu Yamamoto
    • Yoshinari Shinsato
    • Kazunari Arima
    • Toshiyuki Hamada
    • Tatsuhiko Furukawa
  • View Affiliations

  • Published online on: January 24, 2020     https://doi.org/10.3892/ol.2020.11352
  • Pages: 2258-2264
  • Copyright: © Kawahata et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

5‑Fluorouracil (5‑FU) is widely used in the treatment of various types of solid cancer. Our study showed that ribosomal protein L11 (RPL11) was a crucial factor affecting sensitivity of gastric cancer to 5‑FU, implying that RPL11 expression is a potential biomarker for predicting 5‑FU sensitivity. Kaplan‑Meier survival analysis indicated that high RPL11 expression in gastric cancer patients treated with 5‑FU was significantly associated with good prognosis. It was therefore investigated whether RPL11 affected the sensitivity of gastric cancer against 5‑FU using four human gastric cancer cell lines, MKN45 (wild‑type TP53 gene), NUGC4 (wild‑type), MKN7 (mutated), and KE39 cells (mutated). In vitro assays demonstrated that RPL11 knockdown in gastric cancer cell lines carrying the TP53 wild‑type gene attenuated 5‑FU‑induced cell growth suppression and activation of the P53 pathway, but not in cells carrying mutated TP53, suggesting that 5‑FU suppresses tumor progression via RPL11‑mediated activation of the P53 pathway in gastric cancer. The present study provides a potential therapeutic strategy for improving 5‑FU resistance in gastric cancer by elevating RPL11 expression.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Kawahata, T., Kawahara, K., Shimokawa, M., Sakiyama, A., Shiraishi, T., Minami, K. ... Furukawa, T. (2020). Involvement of ribosomal protein L11 expression in sensitivity of gastric cancer against 5‑FU. Oncology Letters, 19, 2258-2264. https://doi.org/10.3892/ol.2020.11352
MLA
Kawahata, T., Kawahara, K., Shimokawa, M., Sakiyama, A., Shiraishi, T., Minami, K., Yamamoto, M., Shinsato, Y., Arima, K., Hamada, T., Furukawa, T."Involvement of ribosomal protein L11 expression in sensitivity of gastric cancer against 5‑FU". Oncology Letters 19.3 (2020): 2258-2264.
Chicago
Kawahata, T., Kawahara, K., Shimokawa, M., Sakiyama, A., Shiraishi, T., Minami, K., Yamamoto, M., Shinsato, Y., Arima, K., Hamada, T., Furukawa, T."Involvement of ribosomal protein L11 expression in sensitivity of gastric cancer against 5‑FU". Oncology Letters 19, no. 3 (2020): 2258-2264. https://doi.org/10.3892/ol.2020.11352