Open Access

Expression levels and associations of five long non‑coding RNAs in gastric cancer and their clinical significance

  • Authors:
    • Helen Barong Binang
    • Yun‑Shan Wang
    • Marlvin Anemey Tewara
    • Lutao Du
    • Shuang Shi
    • Ning Li
    • Ariston Gabriel Abakundana Nsenga
    • Chuanxin Wang
  • View Affiliations

  • Published online on: January 16, 2020     https://doi.org/10.3892/ol.2020.11311
  • Pages: 2431-2445
  • Copyright: © Binang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is a type of cancer that is commonly diagnosed worldwide due to a lack of early diagnostic, prognostic and therapeutic targets for this disease. The aim of the present study was to examine the expression levels of five long non‑coding RNAs, namely PTPRG antisense RNA 1 (PTPRG‑AS1), forkhead box P4 antisense RNA 1 (FOXP4‑AS1), bladder cancer‑associated transcript 2 (BLACAT2), ZXF2 and upregulated in colorectal cancer (UCC), to study their associations with patient characteristics and assess their prognostic efficacy, in order to determine the possibility of their application as GC biomarkers. The expression levels of long non‑coding RNAs (lncRNAs) were determined by reverse transcription‑quantitative PCR analysis of 61 pairs of GC tissues and adjacent healthy gastric mucosa tissues and GC cell lines. The Chi‑square test was conducted to assess the associations of lncRNA expression levels with clinical characteristics of patients. The effect of UCC on GC cell proliferation was determined using in vitro functional experiments. The prognostic efficacy of FOXP4‑AS1, BLACAT2 and UCC were examined in the Gene Expression Profiling Interactive Analysis database and those of PTPRG‑AS1 were examined in the Kaplan Meier Plot database. Gene alteration frequencies of PTPRG‑AS1 and BLACAT2 in GC were identified using the cBioPortal for Cancer Genomics. PTPRG‑AS1, FOXP4‑AS1, BLACAT2, ZXF2 and UCC were found to be upregulated in GC cell lines and GC tissues compared with adjacent normal tissues. PTPRG‑AS1 and ZXF2 expression levels were associated with the expression status of the cell proliferation marker Ki67. UCC promoted the proliferation of GC cells in vitro and was associated with lymph node metastasis. Increased expression of FOXP4‑AS1 indicated a favorable outcome in terms of disease‑free survival, whereas high expression of PTPRG‑AS1 was associated with poor survival rates for patients in different GC risk groups. BLACAT2 gene mutation was associated with poor disease‑free survival outcome for patients with GC. The results suggest that PTPRG‑AS1, FOXP4‑AS1, BLACAT2, ZXF2 and UCC are potential biomarkers for the detection of GC at the molecular level and may be used as potential targets for GC therapy. The individual roles of these lncRNAs may be utilized for prognostic predictions.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Binang, H.B., Wang, Y., Tewara, M.A., Du, L., Shi, S., Li, N. ... Wang, C. (2020). Expression levels and associations of five long non‑coding RNAs in gastric cancer and their clinical significance. Oncology Letters, 19, 2431-2445. https://doi.org/10.3892/ol.2020.11311
MLA
Binang, H. B., Wang, Y., Tewara, M. A., Du, L., Shi, S., Li, N., Abakundana Nsenga, A. G., Wang, C."Expression levels and associations of five long non‑coding RNAs in gastric cancer and their clinical significance". Oncology Letters 19.3 (2020): 2431-2445.
Chicago
Binang, H. B., Wang, Y., Tewara, M. A., Du, L., Shi, S., Li, N., Abakundana Nsenga, A. G., Wang, C."Expression levels and associations of five long non‑coding RNAs in gastric cancer and their clinical significance". Oncology Letters 19, no. 3 (2020): 2431-2445. https://doi.org/10.3892/ol.2020.11311