Open Access

Identification of circulating tumor DNA using a targeted 545‑gene next generation sequencing panel in patients with gastric cancer

  • Authors:
    • Jing Lan
    • Yaping Lu
    • Yanfang Guan
    • Lianpeng Chang
    • Zhengyuan Yu
    • Haixin Qian
  • View Affiliations

  • Published online on: January 16, 2020     https://doi.org/10.3892/ol.2020.11305
  • Pages: 2251-2257
  • Copyright: © Lan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is characterized by unique genetic aberrations. Some of these mutations may be used to predict tumor prognosis or to guide patient therapy. Cell‑free circulating tumor DNA (ctDNA) has been considered a promising alternative to biopsy to identify genome aberrations. However, no standardized methods to detect ctDNA variations in patients with GC are currently available. In the present study, the targeted sequencing of 545 genes was used to identify somatic alterations in tissues and matched plasma samples of nine patients with GC. Driver gene mutations were detected in matched tissues and plasma ctDNA. The mutated reads concordance rate of ctDNA in GC tissues with matched tissues was 45%. A true positive copy number gain of human epidermal growth factor receptor 2 in plasma from patients with GC was identified. Furthermore, the ctDNA fraction in plasma cell‑free DNA (cfDNA) was positively correlated with metastasis lymph node number and with lactate dehydrogenase level. In conclusion, results from the present study suggested that targeted sequencing of plasma ctDNA may be considered a potential option for the clinical monitoring of GC.

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March 2020
Volume 19 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Lan, J., Lu, Y., Guan, Y., Chang, L., Yu, Z., & Qian, H. (2020). Identification of circulating tumor DNA using a targeted 545‑gene next generation sequencing panel in patients with gastric cancer. Oncology Letters, 19, 2251-2257. https://doi.org/10.3892/ol.2020.11305
MLA
Lan, J., Lu, Y., Guan, Y., Chang, L., Yu, Z., Qian, H."Identification of circulating tumor DNA using a targeted 545‑gene next generation sequencing panel in patients with gastric cancer". Oncology Letters 19.3 (2020): 2251-2257.
Chicago
Lan, J., Lu, Y., Guan, Y., Chang, L., Yu, Z., Qian, H."Identification of circulating tumor DNA using a targeted 545‑gene next generation sequencing panel in patients with gastric cancer". Oncology Letters 19, no. 3 (2020): 2251-2257. https://doi.org/10.3892/ol.2020.11305