Open Access

The association between high mobility group box 1 chromatin protein and mitotic chromosomes in glioma cells

  • Authors:
    • Liyun Jia
    • Huiling Song
    • Wange Fan
    • Yanan Song
    • Gang Wang
    • Xueli Li
    • Ying He
    • Anhui Yao
  • View Affiliations

  • Published online on: December 2, 2019     https://doi.org/10.3892/ol.2019.11170
  • Pages: 745-752
  • Copyright: © Jia et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

High mobility group box 1 (HMGB1) is an abundant non‑histone nuclear protein that functions as a structural protein of chromatin, regulating genome replication and recombination, mRNA transcription and DNA repair. HMGB1 has been implicated in the tumorigenesis of various cancer types, and the upregulation of HMGB1 has been demonstrated in glioma cells. However, the association between HMGB1 and the mitotic chromosomes in glioma remains uncharacterized. In the present study, the sub‑cellular localization of HMGB1 in glioma tissues and cells was investigated. In addition, enhanced green fluorescent protein (EGFP)‑tagging of the human HMGB1 protein and chromosome spreading were used to investigate the combination of HMGB1 with mitotic chromosomes. The results of the current study indicated that HMGB1 was localized to the nucleus and the cytoplasm, and it was determined to combine with the condensed chromosomes of proliferating cells in paraformaldehyde (PFA)‑fixed glioma tissues. However, HMGB1 was also associated with interphase (but not mitotic chromosomes) when fixed with chilled methanol and 5% (v/v) acetic acid or PFA in vitro. Data from live cell imaging and chromosome spreading indicated the association of HMGB1 with mitotic chromosomes in glioma cells. The present results suggest that HMGB1 combines with mitotic chromosomes in glioma cells, and that the use of fixatives may result in the dissociation of the HMGB1‑DNA interaction. Therefore, in live specimens and chromosome spreads, EGFP fusion proteins may represent an accurate indicator for the determination of the correct localization and interaction of HMGB1 in glioma cells.

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January 2020
Volume 19 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Jia, L., Song, H., Fan, W., Song, Y., Wang, G., Li, X. ... Yao, A. (2020). The association between high mobility group box 1 chromatin protein and mitotic chromosomes in glioma cells. Oncology Letters, 19, 745-752. https://doi.org/10.3892/ol.2019.11170
MLA
Jia, L., Song, H., Fan, W., Song, Y., Wang, G., Li, X., He, Y., Yao, A."The association between high mobility group box 1 chromatin protein and mitotic chromosomes in glioma cells". Oncology Letters 19.1 (2020): 745-752.
Chicago
Jia, L., Song, H., Fan, W., Song, Y., Wang, G., Li, X., He, Y., Yao, A."The association between high mobility group box 1 chromatin protein and mitotic chromosomes in glioma cells". Oncology Letters 19, no. 1 (2020): 745-752. https://doi.org/10.3892/ol.2019.11170