Open Access

lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF‑β pathway

  • Authors:
    • Bin Dai
    • Zhiyong Xiao
    • Beibei Mao
    • Guangtong Zhu
    • Hui Huang
    • Feng Guan
    • Haiyang Su
    • Zhenyang Lin
    • Weicheng Peng
    • Zhiqiang Hu
  • View Affiliations

  • Published online on: September 25, 2019     https://doi.org/10.3892/ol.2019.10918
  • Pages: 5923-5929
  • Copyright: © Dai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The long noncoding RNA (lncRNA) AWPPH, also termed microRNA‑4435‑2HG, has been associated with the poor prognosis of patients with hepatocellular carcinoma (HCC), and has been demonstrated to promote the progression of HCC and bladder cancer. The present study aimed to investigate the role of lncRNA AWPPH in glioma. The expression levels of AWPPH in tumor tissues obtained from patients with glioma and in plasma samples obtained from patients with glioma and healthy controls were detected by reverse transcription‑quantitative polymerase chain reaction. The plasma levels of transforming growth factor (TGF)‑β1 were measured by an enzyme‑linked immunosorbent assay. An AWPPH expression vector was transfected into human glioma cell lines. Subsequently, cancer cell migration and invasion were assessed by Transwell migration and invasion assays, respectively. The expression of TGF‑β1 in the transfected‑glioma cells was detected by western blot analysis. It was identified that AWPPH expression levels in tumor tissues were higher in patients with metastatic glioma; however, no significant differences in AWPPH expression were revealed between patients with different tumor sizes. The plasma levels of AWPPH were positively correlated with the plasma levels of TGF‑β1 in patients with glioma but not in healthy controls. In addition, AWPPH overexpression enhanced cancer cell migration and invasion, and upregulated TGF‑β1 expression. Treatment with TGF‑β1 demonstrated no significant effect on AWPPH expression; however, a TGF‑β inhibitor attenuated the effects of AWPPH overexpression on cell migration and invasion. Therefore, the present study proposed that AWPPH may promote the migration and invasion of glioma cells by activating the TGF‑β pathway.

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December 2019
Volume 18 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Copy and paste a formatted citation
APA
Dai, B., Xiao, Z., Mao, B., Zhu, G., Huang, H., Guan, F. ... Hu, Z. (2019). lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF‑β pathway. Oncology Letters, 18, 5923-5929. https://doi.org/10.3892/ol.2019.10918
MLA
Dai, B., Xiao, Z., Mao, B., Zhu, G., Huang, H., Guan, F., Su, H., Lin, Z., Peng, W., Hu, Z."lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF‑β pathway". Oncology Letters 18.6 (2019): 5923-5929.
Chicago
Dai, B., Xiao, Z., Mao, B., Zhu, G., Huang, H., Guan, F., Su, H., Lin, Z., Peng, W., Hu, Z."lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF‑β pathway". Oncology Letters 18, no. 6 (2019): 5923-5929. https://doi.org/10.3892/ol.2019.10918