Open Access

MicroRNA‑9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A‑2

  • Authors:
    • Fei Xue
    • Yuntian Liang
    • Zhenrong Li
    • Yanhui Liu
    • Hongwei Zhang
    • Yu Wen
    • Lei Yan
    • Qiang Tang
    • Erhui Xiao
    • Dongyi Zhang
  • View Affiliations

  • Published online on: November 14, 2017     https://doi.org/10.3892/ol.2017.7399
  • Copyright: © Xue et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Hepatocellular carcinoma (HCC) is one of the most widespread malignant human tumors worldwide. Treatment options include radiotherapy, surgical intervention and chemotherapy; however, drug resistance is an ongoing treatment concern. In the present study, the effects of a microRNA (miR/miRNA), miR‑9, on the sensitivity of HCC cell lines to the epidermal growth factor receptor inhibitor, cetuximab, were examined. miR‑9 has been proposed to serve a role in tumorigenesis and tumor progression. In the present study, bioinformatics analyses identified the eukaryotic translation initiation factor 5A2 (eIF‑5A‑2) as a target of miR‑9. The expression levels of miR‑9 and eIF‑5A‑2 were examined by reverse transcription‑quantitative polymerase chain reaction and HCC cell lines were transfected with miR‑9 mimics and inhibitors to determine the effects of the miRNA on cell proliferation and viability. The miR‑9 mimic was revealed to significantly increase the sensitivity of epithelial phenotype HCC cells (Hep3B and Huh7) to cetuximab, while the miR‑9 inhibitor triggered the opposite effect. There were no significant differences in sensitivity to cetuximab observed in mesenchymal phenotype HCC cells (SNU387 and SNU449). Cells lines displaying high expression levels of eIF‑5A‑2 were more resistant to cetuximab. Transfection of cells with a miR‑9 mimic resulted in downregulation of the expression of eIF‑5A‑2 mRNA, while an miR‑9 inhibitor increased expression. When expression of eIF‑5A‑2 was knocked down with siRNA, the effects of miR‑9 on cetuximab sensitivity were no longer observed. Taken together, these data support a role for miR‑9 in enhancing the sensitivity of epithelial phenotype HCC cells to cetuximab through regulation of eIF‑5A‑2.

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Print ISSN: 1792-1074
Online ISSN:1792-1082

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APA
Xue, F., Liang, Y., Li, Z., Liu, Y., Zhang, H., Wen, Y. ... Zhang, D. (1899). MicroRNA‑9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A‑2. Oncology Letters, 0, 0-0. https://doi.org/10.3892/ol.2017.7399
MLA
Xue, F., Liang, Y., Li, Z., Liu, Y., Zhang, H., Wen, Y., Yan, L., Tang, Q., Xiao, E., Zhang, D."MicroRNA‑9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A‑2". Oncology Letters 0.0 (1899): 0-0.
Chicago
Xue, F., Liang, Y., Li, Z., Liu, Y., Zhang, H., Wen, Y., Yan, L., Tang, Q., Xiao, E., Zhang, D."MicroRNA‑9 enhances sensitivity to cetuximab in epithelial phenotype hepatocellular carcinoma cells through regulation of the eukaryotic translation initiation factor 5A‑2". Oncology Letters 0, no. 0 (1899): 0-0. https://doi.org/10.3892/ol.2017.7399