Open Access

CircRNA expression profiles in human visceral preadipocytes and adipocytes

  • Authors:
    • Wenxing Sun
    • Xuecheng Sun
    • Weiwei Chu
    • Shigang Yu
    • Fulu Dong
    • Guangfei Xu
  • View Affiliations

  • Published online on: December 16, 2019     https://doi.org/10.3892/mmr.2019.10886
  • Pages: 815-821
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Circular RNAs (circRNAs) regulate several physiological and pathological processes, but their role in visceral lipid deposition has not been explored. In the present study, human preadipocytes from visceral fat tissue (HPA‑v) were induced to form adipocytes, and the circRNA expression profiles in HPA‑v and adipocytes were detected using circRNA microarrays. The microarray data revealed that 2,215 and 1,865 circRNAs were significantly up‑ and downregulated, respectively, in adipocytes compared with HPA‑v. Moreover, the parental genes of differentially expressed circRNAs were associated with fatty acid metabolism based on Kyoto Encyclopedia of Genes and Genomes analysis. Three circRNAs (hsa_circ_0136134, hsa_circ_0017650, and hsa‑circRNA9227‑1) were selected for quantitative PCR (qPCR) validation, and the qPCR results were consistent with the microarray results. Furthermore, MiRanda software was used to predict the microRNAs (miRNAs) potentially targeting the top 10 up‑ and downregulated circRNAs, and 14 miRNAs with more than two miRNA response elements targeting these circRNAs. This is the first study of the expression profiles of circRNAs in HPA‑v and adipocytes and may suggest potential therapeutic targets for the visceral obesity.
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February 2020
Volume 21 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Sun, W., Sun, X., Chu, W., Yu, S., Dong, F., & Xu, G. (2020). CircRNA expression profiles in human visceral preadipocytes and adipocytes. Molecular Medicine Reports, 21, 815-821. https://doi.org/10.3892/mmr.2019.10886
MLA
Sun, W., Sun, X., Chu, W., Yu, S., Dong, F., Xu, G."CircRNA expression profiles in human visceral preadipocytes and adipocytes". Molecular Medicine Reports 21.2 (2020): 815-821.
Chicago
Sun, W., Sun, X., Chu, W., Yu, S., Dong, F., Xu, G."CircRNA expression profiles in human visceral preadipocytes and adipocytes". Molecular Medicine Reports 21, no. 2 (2020): 815-821. https://doi.org/10.3892/mmr.2019.10886