Open Access

Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells

  • Authors:
    • Chun Rao
    • Yi‑Ran Ni
    • Yan‑Min Zhao
    • Yan‑Qiong Zhang
    • Rui‑Ting Zhou
    • Chang‑Bai Liu
    • Lin Han
    • Jiang‑Feng Wu
  • View Affiliations

  • Published online on: December 13, 2019     https://doi.org/10.3892/mmr.2019.10881
  • Pages: 667-674
  • Copyright: © Rao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The aim of the present study was to investigate whether class C1 decoy oligodeoxynucleotides (ODNs) can inhibit the expression of pro‑fibrotic genes associated with rat hepatic stellate cell (HSC) activation and hepatic fibrosis. Luciferase reporter assays were performed to test the promoter activities of transforming growth factor (TGF)‑β and its downstream target genes following transfection of decoy ODNs and plasmids into HSC‑T6 cells, and western blot assays were performed to measure the protein expression of those genes following decoy ODN transfection. Class C1 decoy ODNs were confirmed to inhibit the promoter activity of TGF‑β and its downstream target genes, such as type 1 collagen (COLI)α1, tissue inhibitor of metalloproteinases (TIMP)1 and α‑smooth muscle actin by Gaussia luciferase reporter assay, and to further downregulate the expression of TGF‑β, SMAD3, COLIα1 and TIMP1 by western blotting in activated HSC‑T6 cells. In conclusion, class C1 decoy ODNs inhibited pro‑fibrotic gene expression in rat HSCS by downregulating TGF‑β signaling.
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February 2020
Volume 21 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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APA
Rao, C., Ni, Y., Zhao, Y., Zhang, Y., Zhou, R., Liu, C. ... Wu, J. (2020). Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells. Molecular Medicine Reports, 21, 667-674. https://doi.org/10.3892/mmr.2019.10881
MLA
Rao, C., Ni, Y., Zhao, Y., Zhang, Y., Zhou, R., Liu, C., Han, L., Wu, J."Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells". Molecular Medicine Reports 21.2 (2020): 667-674.
Chicago
Rao, C., Ni, Y., Zhao, Y., Zhang, Y., Zhou, R., Liu, C., Han, L., Wu, J."Class C1 decoy oligodeoxynucleotide inhibits profibrotic genes expression in rat hepatic stellate cells". Molecular Medicine Reports 21, no. 2 (2020): 667-674. https://doi.org/10.3892/mmr.2019.10881