Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells

  • Authors:
    • Zuofei Chi
    • Hongyu Gao
    • Hui Liu
    • Bin Wu
    • Bin Zhang
    • Min Gu
    • Wei Yang
  • View Affiliations

  • Published online on: December 10, 2019     https://doi.org/10.3892/mmr.2019.10873
  • Pages: 936-944
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

T‑cell acute lymphoblastic leukemia (T‑ALL) is a hematopoietic malignancy, which is associated with a poor prognosis. It is difficult to achieve complete remission or long‑term survival with conventional chemotherapy, partly due to decreased apoptosis. However, necroptosis can serve as an alternative pathway to induce cell death. The present study investigated whether the selective histone deacetylase (HDAC) inhibitor chidamide exerted a therapeutic effect on T‑ALL and explored the underlying mechanism. The results revealed that HDAC expression was increased in Jurkat and HUT‑78 cells treated compared with the control cell line (H9), and was accompanied by elevated cellular Fas‑associated death domain‑like interleukin‑1β converting enzyme inhibitory protein long form (c‑FLIPL) levels. Chidamide treatment (2 µmol/l) also induced mitochondrial dysfunction, necroptosis and apoptosis in T‑ALL cells in vitro. Furthermore, necroptosis was increased when apoptosis was blocked in T‑ALL cells. Additionally, chidamide (2 µmol/l) downregulated c‑FLIPL, HDAC1 and HDAC3 expression, and increased receptor‑interacting protein kinase 3 expression and the phosphorylation of mixed lineage kinase domain‑like pseudokinase in Jurkat and HUT‑78 cells. The results obtained in the present study revealed that chidamide may induce necroptosis via regulation of c‑FLIPL expression when apoptosis is inhibited in Jurkat and HUT‑78 cells.
View Figures
View References

Related Articles

Journal Cover

February 2020
Volume 21 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
APA
Chi, Z., Gao, H., Liu, H., Wu, B., Zhang, B., Gu, M., & Yang, W. (2020). Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells. Molecular Medicine Reports, 21, 936-944. https://doi.org/10.3892/mmr.2019.10873
MLA
Chi, Z., Gao, H., Liu, H., Wu, B., Zhang, B., Gu, M., Yang, W."Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells". Molecular Medicine Reports 21.2 (2020): 936-944.
Chicago
Chi, Z., Gao, H., Liu, H., Wu, B., Zhang, B., Gu, M., Yang, W."Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells". Molecular Medicine Reports 21, no. 2 (2020): 936-944. https://doi.org/10.3892/mmr.2019.10873