Optimal conditions for the storage of German cockroach extract

  • Authors:
    • Kyoung Yong Jeong
    • Jongsun Lee
    • Ji‑Eun Yuk
    • Kyung Hee Park
    • Jae‑Hyun Lee
    • Jung Dong Kim
    • Jung‑Won Park
  • View Affiliations

  • Published online on: November 27, 2019     https://doi.org/10.3892/mmr.2019.10854
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Abstract

Allergen extracts are commonly utilized for diagnosis and immunotherapy; however, the stability of protease‑rich extracts is important for a precise diagnosis and treatment efficacy. The present study determines the optimal conditions for the storage of German cockroach allergen extract. Cockroach extracts were reconstituted in four buffers: normal saline (NS), 50% glycerol in NS, 0.3% phenol in NS, or 0.3% phenol and 50% glycerol in NS. The extracts in different buffers were stored either at room temperature (18‑26˚C, RT) or refrigerated (2‑8˚C). Subsequently, the protein concentration and allergen content (Bla g 1 and Bla g 2) in the extracts were examined for the course of one year. Extract potency was estimated by inhibition ELISA. At least 90.5% protein, 94.4% Bla g 1, 65.2% Bla g 2, and 91.4% potency remained after one year when 50% glycerol NS was added to the extract with refrigeration. However, less than 13.7% protein, 17.1% Bla g 1, 0% Bla g 2 and 32.5% potency were maintained after one year when 50% glycerol NS was not added to the extract and was maintained at RT. The addition of 0.3% phenol NS did not show significant effects on extract stability. The addition of 50% glycerol NS and refrigerated storage temperature were found to be important factors for increasing the shelf life of protease‑rich cockroach extract.

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APA
Jeong, K.Y., Lee, J., Yuk, J., Park, K.H., Lee, J., Kim, J.D., & Park, J. (1899). Optimal conditions for the storage of German cockroach extract. Molecular Medicine Reports, 0, 0-0. https://doi.org/10.3892/mmr.2019.10854
MLA
Jeong, K. Y., Lee, J., Yuk, J., Park, K. H., Lee, J., Kim, J. D., Park, J."Optimal conditions for the storage of German cockroach extract". Molecular Medicine Reports 0.0 (1899): 0-0.
Chicago
Jeong, K. Y., Lee, J., Yuk, J., Park, K. H., Lee, J., Kim, J. D., Park, J."Optimal conditions for the storage of German cockroach extract". Molecular Medicine Reports 0, no. 0 (1899): 0-0. https://doi.org/10.3892/mmr.2019.10854