Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells

  • Authors:
    • Ting‑Wen Chung
    • Yi‑Rong Li
    • Wei Yuan Huang
    • Jui‑Hsin Su
    • Hong‑Lin Chan
    • Sheng‑Hao Lin
    • Chin‑San Liu
    • Shih‑Chao Lin
    • Chi‑Chien Lin
    • Ching‑Hsiung Lin
  • View Affiliations

  • Published online on: September 13, 2017     https://doi.org/10.3892/mmr.2017.7480
  • Pages:6992-7000
0

Abstract

The dendritic cell (DC) maturation process is essential for the development of T cell responses and immune tolerance. Accordingly, DCs are considered a major target in the development of immunomodulating agents. In the present study, the effect of sinulariolide, an active compound isolated from the cultured soft coral Sinularia flexibilis, on lipopolysaccharide (LPS)‑induced murine bone marrow‑derived DCs was evaluated. The different phenotypes, cytokine secretion and the mix‑lymphocyte reaction of DCs were detected using flow cytometry and ELISA. The experimental results revealed that the phenotypic and functional maturation of DCs stimulated by LPS were markedly reduced by sinulariolide in a concentration‑dependent manner, including the expression of co‑stimulatory molecules (CD40, CD80 and CD86). In addition, sinulariolide reduced the release of tumor necrosis factor‑α, interleukin (IL)‑6, IL‑12 and nitric oxide from the LPS‑activated DCs, decreased their abilities to stimulate allogeneic T cell proliferation, and inhibited LPS‑induced nuclear factor‑κB pathways. These findings offer novel insight into the immunopharmacological function of sinulariolide and its effects on DCs.

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November 2017
Volume 16 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

2016 Impact Factor: 1.692
Ranked #19/128 Medicine Research and Experimental
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APA
Chung, T., Li, Y., Huang, W., Su, J., Chan, H., Lin, S. ... Lin, C. (2017). Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells. Molecular Medicine Reports, 16, 6992-7000. https://doi.org/10.3892/mmr.2017.7480
MLA
Chung, T., Li, Y., Huang, W., Su, J., Chan, H., Lin, S., Liu, C., Lin, S., Lin, C., Lin, C."Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells". Molecular Medicine Reports 16.5 (2017): 6992-7000.
Chicago
Chung, T., Li, Y., Huang, W., Su, J., Chan, H., Lin, S., Liu, C., Lin, S., Lin, C., Lin, C."Sinulariolide suppresses LPS‑induced phenotypic and functional maturation of dendritic cells". Molecular Medicine Reports 16, no. 5 (2017): 6992-7000. https://doi.org/10.3892/mmr.2017.7480