High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma

  • Authors:
    • Jian Qin
    • Min Wang
    • Jun Qin
    • Qu Cai
    • Zhi‑Hai Peng
  • View Affiliations

  • Published online on: August 16, 2018     https://doi.org/10.3892/mco.2018.1700
  • Pages: 467-471
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Conditionally replicating adenoviral vectors constructed with tumor‑specific promoters (TSPs) offers a viable tool for the treatment of cholangiocarcinoma. The aim of the present study was to investigate cholangiocarcinoma‑specific TSPs that remain active in adenoviral constructs in gene therapy. The mRNA expressions of cyclooxygenase‑2, cytokeratin‑19 (CK19), mucin‑1, midkine and telomerase reverse transcriptase were determined in human cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using reverse transcription‑quantitative polymerase chain reaction. The candidate promoters constructed in adenoviral vectors were analyzed for their activities in cholangiocarcinoma cell lines, primary human hepatocytes and cholangiocytes using dual‑luciferase reporter assays. The mRNA expression of CK19 was markedly higher in the QBC939 cell line, indicating specificity to cholangiocarcinoma. Moreover, the promoter activity of CK19 in the adenoviral vector in infected cholangiocarcinoma cells was found to be significantly stronger compared with that in infected primary human hepatocytes and cholangiocytes. CK19 may be implicated in the pathogenesis of cholangiocarcinoma, as demonstrated by the stronger activity of its promoter, as well as the higher expression of mRNA in tumor cells. Therefore, the use of the promoter sequence of the CK19 gene may represent a potential tool in cholangiocarcinoma‑specific adenoviral gene therapy.
View Figures
View References

Related Articles

Journal Cover

October 2018
Volume 9 Issue 4

Print ISSN: 2049-9450
Online ISSN:2049-9469

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Qin, J., Wang, M., Qin, J., Cai, Q., & Peng, Z. (2018). High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma. Molecular and Clinical Oncology, 9, 467-471. https://doi.org/10.3892/mco.2018.1700
MLA
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9.4 (2018): 467-471.
Chicago
Qin, J., Wang, M., Qin, J., Cai, Q., Peng, Z."High promoter activity of cytokeratin‑19 gene in cholangiocarcinoma". Molecular and Clinical Oncology 9, no. 4 (2018): 467-471. https://doi.org/10.3892/mco.2018.1700