One-step nucleic acid amplification assay for intraoperative prediction of advanced axillary lymph node metastases in breast cancer patients with sentinel lymph node metastasis

  • Authors:
    • Michiyo Kubota
    • Yoshifumi Komoike
    • Mika Hamada
    • Wataru Shinzaki
    • Tatsuya Azumi
    • Yukihiko Hashimoto
    • Shigeru Imoto
    • Yoshifumi Takeyama
    • Kiyotaka Okuno
  • View Affiliations

  • Published online on: December 7, 2015     https://doi.org/10.3892/mco.2015.694
  • Pages: 173-178
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Abstract

The one-step nucleic acid amplification (OSNA) assay is used to semiquantitatively measure the cytokeratin (CK)19 mRNA copy numbers of each sentinel lymph node (SLN) in breast cancer patients. The aim of the present study was to evaluate whether the diagnosis of ≥4 LN metastases is possible using the OSNA assay intraoperatively. Between May, 2010 and December, 2014, a total of 134 patients who underwent axillary lymph node dissection (ALND) of positive SLNs were analyzed. The total tumor load (TTL) was defined as the total CK19 mRNA copies of all positive SLNs. the correlation between TTL and ≥4 LN metastases was evaluated. Of the 134 patients, 31 (23.1%) had ≥4 LN metastases. TTL ≥5.4x104 copies/µl evaluated by receiver operator characteristic curve analysis was examined along with other clinicopathological variables. In the multivariate analysis, only TTL ≥5.4x104 copies/µl was correlated with ≥4 LN metastases (odds ratio = 2.95, 95% confidence interval: 1.17‑7.97, P=0.022). Therefore, TTL assessed by the OSNA assay has the potential to be a predictor of ≥4 LN metastases and it may be useful for the selection of patients with positive SLNs in whom ALND may be safely omitted.
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February 2016
Volume 4 Issue 2

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APA
Kubota, M., Komoike, Y., Hamada, M., Shinzaki, W., Azumi, T., Hashimoto, Y. ... Okuno, K. (2016). One-step nucleic acid amplification assay for intraoperative prediction of advanced axillary lymph node metastases in breast cancer patients with sentinel lymph node metastasis. Molecular and Clinical Oncology, 4, 173-178. https://doi.org/10.3892/mco.2015.694
MLA
Kubota, M., Komoike, Y., Hamada, M., Shinzaki, W., Azumi, T., Hashimoto, Y., Imoto, S., Takeyama, Y., Okuno, K."One-step nucleic acid amplification assay for intraoperative prediction of advanced axillary lymph node metastases in breast cancer patients with sentinel lymph node metastasis". Molecular and Clinical Oncology 4.2 (2016): 173-178.
Chicago
Kubota, M., Komoike, Y., Hamada, M., Shinzaki, W., Azumi, T., Hashimoto, Y., Imoto, S., Takeyama, Y., Okuno, K."One-step nucleic acid amplification assay for intraoperative prediction of advanced axillary lymph node metastases in breast cancer patients with sentinel lymph node metastasis". Molecular and Clinical Oncology 4, no. 2 (2016): 173-178. https://doi.org/10.3892/mco.2015.694