Plasma miR‑200c and miR‑18a as potential biomarkers for the detection of colorectal carcinoma

  • Authors:
    • Guang-Jun Zhang
    • Tong Zhou
    • Zuo-Liang Liu
    • Hong-Peng Tian
    • Shu-Sen Xia
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  • Published online on: January 7, 2013     https://doi.org/10.3892/mco.2013.61
  • Pages: 379-384
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Abstract

It has been demonstrated that there are abundant stable microRNAs (miRNAs) in plasma/serum, which can be detected and are potentially disease‑specific. The aim of this study was to investigate whether plasma miR‑200c and miR‑18a can be used as biomarkers for the detection of colorectal carcinoma (CRC). This study was divided into three parts: i) confirmation of higher miR‑200c and miR‑18a levels in primary CRC tissues compared to normal colorectal tissues; ii) evaluation of plasma miR‑200c and miR‑18a expression by comparing 78 patients with 86 healthy volunteers and iii) comparison of miR‑200c and miR‑18a levels in paired pre-and post-operative plasma in cancer patients who underwent curative CRC resection. Results showed that the expression of miR‑200c and miR‑18a was significantly higher in CRC compared to normal tissues. The plasma levels of miR‑200c and miR‑18a were significantly higher in CRC patients compared to controls. miR‑200c yielded an area under the receiver‑operating characteristics (ROC) curve (AUC) of 0.749 and miR‑18a yielded an AUC of 0.804 when distinguishing CRC patients from the controls. Combined ROC analyses using the two miRNAs revealed an elevated AUC of 0.839 with 84.6% sensitivity and 75.6% specificity in discriminating CRC. Plasma levels of miR‑200c and miR‑18a were significantly lower in post‑operative compared to pre‑operative samples. The results of this study suggest that plasma miR‑200c and miR‑18a are significantly elevated in the plasma of CRC patients and that they may serve as non-invasive molecular markers for CRC screening.
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March 2013
Volume 1 Issue 2

Print ISSN: 2049-9450
Online ISSN:2049-9469

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APA
Zhang, G., Zhou, T., Liu, Z., Tian, H., & Xia, S. (2013). Plasma miR‑200c and miR‑18a as potential biomarkers for the detection of colorectal carcinoma. Molecular and Clinical Oncology, 1, 379-384. https://doi.org/10.3892/mco.2013.61
MLA
Zhang, G., Zhou, T., Liu, Z., Tian, H., Xia, S."Plasma miR‑200c and miR‑18a as potential biomarkers for the detection of colorectal carcinoma". Molecular and Clinical Oncology 1.2 (2013): 379-384.
Chicago
Zhang, G., Zhou, T., Liu, Z., Tian, H., Xia, S."Plasma miR‑200c and miR‑18a as potential biomarkers for the detection of colorectal carcinoma". Molecular and Clinical Oncology 1, no. 2 (2013): 379-384. https://doi.org/10.3892/mco.2013.61