Expression of the breast cancer resistance protein and 5‑fluorouracil resistance in clinical breast cancer tissue specimens

  • Authors:
    • Min Wang
    • Xianming Wang
    • Jianhui Yuan
    • Liangfeng Guo
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  • Published online on: July 4, 2013     https://doi.org/10.3892/mco.2013.143
  • Pages: 853-857
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Abstract

The breast cancer resistance protein (BCRP) is a recently characterized xenobiotic half‑transporter protein that acts as an energy‑dependent efflux pump and may be associated with the multidrug‑resistant phenotype. The aim of this study was to determine the association between BCRP expression and 5‑fluorouracil (5‑Fu) resistance in clinical breast cancer tissue specimens. The BCRP expression was investigated using quantitative reverse‑transcriptase polymerase chain reaction (RT‑PCR) by use of the Master SYBR‑Green I reagent and immunohistochemistry (IHC) by use of the BXP‑21 anti‑BCRP monoclonal antibody in clinical breast cancer tissue specimens. Chemosensitivity to 5‑Fu for BCRP‑positive clinical breast cancer tissue specimens was colorimetrically assessed with the cytotoxicity assay through methyl thiazolyl tetrazolium (MTT) reduction. A total of 37 BCRP‑positive clinical breast cancer tissue specimens were identified with quantitative RT‑PCR and IHC. There was a significant correlation in BCRP expression between the results of quantitative RT‑PCR and IHC in the specimens. the fold resistance to 5‑Fu was 7‑12 compared to sensitivity to paclitaxel as determined by the colorimetric assay through MTT reduction in the 37 specimens. Our study results indicated that 5‑Fu resistance may be mediated by BCRP expression in clinical breast cancer tissue specimens, which may help optimize the design of breast cancer clinical chemotherapy schemes in BCRP‑positive specimens.
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September 2013
Volume 1 Issue 5

Print ISSN: 2049-9450
Online ISSN:2049-9469

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APA
Wang, M., Wang, X., Yuan, J., & Guo, L. (2013). Expression of the breast cancer resistance protein and 5‑fluorouracil resistance in clinical breast cancer tissue specimens. Molecular and Clinical Oncology, 1, 853-857. https://doi.org/10.3892/mco.2013.143
MLA
Wang, M., Wang, X., Yuan, J., Guo, L."Expression of the breast cancer resistance protein and 5‑fluorouracil resistance in clinical breast cancer tissue specimens". Molecular and Clinical Oncology 1.5 (2013): 853-857.
Chicago
Wang, M., Wang, X., Yuan, J., Guo, L."Expression of the breast cancer resistance protein and 5‑fluorouracil resistance in clinical breast cancer tissue specimens". Molecular and Clinical Oncology 1, no. 5 (2013): 853-857. https://doi.org/10.3892/mco.2013.143