Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma

  • Authors:
    • Nijiro Nohata
    • Toyoyuki Hanazawa
    • Naoko Kikkawa
    • Muradil Mutallip
    • Lisa Fujimura
    • Hirofumi Yoshino
    • Kazumori Kawakami
    • Takeshi Chiyomaru
    • Hideki Enokida
    • Masayuki Nakagawa
    • Yoshitaka Okamoto
    • Naohiko Seki
  • View Affiliations

  • Published online on: January 1, 2011     https://doi.org/10.3892/ijo_00000840
  • Pages: 209-217
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Abstract

MicroRNAs (miRNAs) are small non-coding RNAs of approximately 22 nucleotides that can function as oncogenes or tumor suppressors in human cancer. Down-regulation of the miRNA miR-133a in many type of cancers, and a reduction of cell proliferation, migration, and invasion upon over-expression, suggests that miR-133a is a tumor suppressor. In this study, genome-wide gene expression analysis of HNSCC cells that over-express miR-133a showed that caveolin-1 (CAV1), a multifunctional scaffolding protein, is down-regulated, a result that was confirmed by real-time PCR and Western blot analysis. A luciferase reporter assay revealed that miR-133a is directly bound to CAV1 mRNA. Cancer cell migration and invasion were significantly inhibited in HNSCC cells transfected with si-CAV1. Therefore, CAV1 functions as an oncogene in HNSCC. The identification of tumor suppressive miRNAs and their target genes could provide new insights into potential mechanism of HNSCC carcinogenesis.

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January 2011
Volume 38 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Nohata, N., Hanazawa, T., Kikkawa, N., Mutallip, M., Fujimura, L., Yoshino, H. ... Seki, N. (2011). Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma. International Journal of Oncology, 38, 209-217. https://doi.org/10.3892/ijo_00000840
MLA
Nohata, N., Hanazawa, T., Kikkawa, N., Mutallip, M., Fujimura, L., Yoshino, H., Kawakami, K., Chiyomaru, T., Enokida, H., Nakagawa, M., Okamoto, Y., Seki, N."Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma". International Journal of Oncology 38.1 (2011): 209-217.
Chicago
Nohata, N., Hanazawa, T., Kikkawa, N., Mutallip, M., Fujimura, L., Yoshino, H., Kawakami, K., Chiyomaru, T., Enokida, H., Nakagawa, M., Okamoto, Y., Seki, N."Caveolin-1 mediates tumor cell migration and invasion and its regulation by miR-133a in head and neck squamous cell carcinoma". International Journal of Oncology 38, no. 1 (2011): 209-217. https://doi.org/10.3892/ijo_00000840