Leucine-rich protein 130 contributes to apoptosis resistance of human hepatocarcinoma cells

  • Authors:
    • Mickaël Michaud
    • Stéphane Barakat
    • Sandrine Magnard
    • Dominique Rigal
    • Loris G. Baggetto
  • View Affiliations

  • Published online on: January 1, 2011     https://doi.org/10.3892/ijo_00000836
  • Pages: 169-178
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

LRP130 is a ubiquitous protein involved in cellular homeostasis, microtubule alteration, and transactivation of a few multidrug resistance genes. Its role in resistance to apoptosis in HepG2 and HUH7 hepatocarcinoma cells was investigated. Using shRNA-producing lentiviruses to down-regulate the LRP130 gene, we showed that i) LRP130 did not affect the capacity of hepatocarcinoma cells to extrude drugs since LRP130 down-regulation was insufficient to significantly reduce P-glycoprotein production in these cells, and ii) the expression of 11 apoptosis-related genes measured by PCR-array was significantly reduced. Interestingly, six of these genes encode extrinsic pathway proapoptotic proteins whose expression was higher in LRP130-non producing than in LRP130-producing HepG2 cells. Fluorescence microscopy confirmed this new anti-apoptotic role of LRP130, which is strengthened by a significantly reduced cytochrome c oxidase activity in LRP130-down-regulated hepatocarcinoma cells.

Related Articles

Journal Cover

January 2011
Volume 38 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Michaud, M., Barakat, S., Magnard, S., Rigal, D., & Baggetto, L.G. (2011). Leucine-rich protein 130 contributes to apoptosis resistance of human hepatocarcinoma cells. International Journal of Oncology, 38, 169-178. https://doi.org/10.3892/ijo_00000836
MLA
Michaud, M., Barakat, S., Magnard, S., Rigal, D., Baggetto, L. G."Leucine-rich protein 130 contributes to apoptosis resistance of human hepatocarcinoma cells". International Journal of Oncology 38.1 (2011): 169-178.
Chicago
Michaud, M., Barakat, S., Magnard, S., Rigal, D., Baggetto, L. G."Leucine-rich protein 130 contributes to apoptosis resistance of human hepatocarcinoma cells". International Journal of Oncology 38, no. 1 (2011): 169-178. https://doi.org/10.3892/ijo_00000836