Genetic alterations after carbon ion irradiation in human lung adenocarcinoma cells

  • Authors:
    • Emmanouil Fokas
    • An You
    • Janko Juricko
    • Rita Engenhart-Cabillic
    • Han-Xiang An
  • View Affiliations

  • Published online on: January 1, 2011     https://doi.org/10.3892/ijo_00000835
  • Pages: 161-168
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Abstract

The aim of this study was to investigate the difference in gene expression profiles of human lung adenocarcinoma cells and identify genes whose expression is altered by heavy ions but not X-rays. The lung adenocarcinoma cell line A549 was irradiated with carbon ion beams and X-rays using biologically equivalent doses (2 Gy and 6 Gy, respectively). The transcriptional profiling was determined with a high density cDNA microarray containing 11.800 genes, and genetic network and gene ontology analysis was performed. The changes in selected genes involved were validated by quantitative real-time polymerase chain reaction (qRT-PCR). The microarray analysis identified 49 mapped, network-eligible genes, the expression level of which was altered by carbon ions but not by X-rays. From these, 29 were upregulated while 20 genes were downregulated 4 h post-irradiation with carbon ions in A549 cells, as compared to the control. Among these, three genes (CCND2, RARG and E2F5) were involved in the aryl hydrocarbon receptor signalling and G1/S cell cycle checkpoint pathways. The microarray data were corroborated by qRT-PCR analysis of the selected genes (p<0.05). Our findings provide information on the genetic signature of carbon ions in human lung adenocarcinoma cells and add to the understanding of the complicated molecular response to carbon ion irradiation.

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January 2011
Volume 38 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Fokas, E., You, A., Juricko, J., Engenhart-Cabillic, R., & An, H. (2011). Genetic alterations after carbon ion irradiation in human lung adenocarcinoma cells. International Journal of Oncology, 38, 161-168. https://doi.org/10.3892/ijo_00000835
MLA
Fokas, E., You, A., Juricko, J., Engenhart-Cabillic, R., An, H."Genetic alterations after carbon ion irradiation in human lung adenocarcinoma cells". International Journal of Oncology 38.1 (2011): 161-168.
Chicago
Fokas, E., You, A., Juricko, J., Engenhart-Cabillic, R., An, H."Genetic alterations after carbon ion irradiation in human lung adenocarcinoma cells". International Journal of Oncology 38, no. 1 (2011): 161-168. https://doi.org/10.3892/ijo_00000835