Open Access

miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples

  • Authors:
    • M. Rentoft
    • J. Fahlén
    • P. J. Coates
    • G. Laurell
    • B. Sjöström
    • P. Rydén
    • K. Nylander
  • View Affiliations

  • Published online on: January 1, 2011     https://doi.org/10.3892/ijo_00000824
  • Pages: 61-69
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Abstract

Global miRNA expression arrays were used for analysis of 836 miRNAs in formalin-fixed paraffin-embedded samples from 21 tongue cancer patients and 8 controls. Samples had been stored for one to eleven years. Results separated tumour samples from controls, however, the largest variation was correlated to sample storage time, detectable already after one year. With the use of a linear regression model we could adjust for the storage-dependent effect, leading to the identification of 54 differentially expressed miRNAs in tongue cancer, compared to 16 when using standard normalization, including up-regulation of a novel miRNA, miR-424.

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January 2011
Volume 38 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Rentoft, M., Fahlén, J., Coates, P.J., Laurell, G., Sjöström, B., Rydén, P., & Nylander, K. (2011). miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples. International Journal of Oncology, 38, 61-69. https://doi.org/10.3892/ijo_00000824
MLA
Rentoft, M., Fahlén, J., Coates, P. J., Laurell, G., Sjöström, B., Rydén, P., Nylander, K."miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples". International Journal of Oncology 38.1 (2011): 61-69.
Chicago
Rentoft, M., Fahlén, J., Coates, P. J., Laurell, G., Sjöström, B., Rydén, P., Nylander, K."miRNA analysis of formalin-fixed squamous cell carcinomas of the tongue is affected by age of the samples". International Journal of Oncology 38, no. 1 (2011): 61-69. https://doi.org/10.3892/ijo_00000824