Expression of proteinase inhibitor-9/serpinB9 in non-small cell lung carcinoma cells and tissues

  • Authors:
    • Ilona Rousalova
    • Evzen Krepela
    • Jan Prochazka
    • Jan Cermak
    • Kamila Benkova
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijo_00000498
  • Pages: 275-283
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Abstract

Human proteinase inhibitor-9 (PI-9)/serpinB9 is an intracellular ovalbumin-family serpin with nucleocytoplasmic distribution which is expressed in certain normal cell types and cancer cells of different origin. Due to binding and inactivating of granzyme B (GrB), PI-9 can protect the cells from GrB-mediated apoptosis. High levels of PI-9 expression in certain cancer cells may contribute to their resistance against the immune mediated killing. So far, it is not known whether non-small cell lung cardinomas (NSCLCs) express PI-9 mRNA and a functional PI-9 protein. Herein we report for the first time that NSCLC cells express both PI-9 mRNA and protein and that there is a subset of NSCLC cells with upregulated PI-9 mRNA and protein expression. Futhermore, our work revealed that the PI-9 protein expressed in NSCLC cells can inhibit the active GrB. Finally, analysis of PI-9 mRNA expression in NSCLC tumours from surgically treated patients showed that the expression of this transcript is upregulated in the less-differentiated lung adenocarcinomas. We suggest, that the upregulated expression of PI-9 in NSCLC cells may serve to protect them from apoptosis induced by GrB.

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January 2010
Volume 36 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Rousalova, I., Krepela, E., Prochazka, J., Cermak, J., & Benkova, K. (2010). Expression of proteinase inhibitor-9/serpinB9 in non-small cell lung carcinoma cells and tissues. International Journal of Oncology, 36, 275-283. https://doi.org/10.3892/ijo_00000498
MLA
Rousalova, I., Krepela, E., Prochazka, J., Cermak, J., Benkova, K."Expression of proteinase inhibitor-9/serpinB9 in non-small cell lung carcinoma cells and tissues". International Journal of Oncology 36.1 (2010): 275-283.
Chicago
Rousalova, I., Krepela, E., Prochazka, J., Cermak, J., Benkova, K."Expression of proteinase inhibitor-9/serpinB9 in non-small cell lung carcinoma cells and tissues". International Journal of Oncology 36, no. 1 (2010): 275-283. https://doi.org/10.3892/ijo_00000498