Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP

  • Authors:
    • Jing Wen
    • Bin Zheng
    • Yi Hu
    • Xu Zhang
    • Hong Yang
    • Yan Li
    • Chun-Yu Zhang
    • Kong-Jia Luo
    • Xing Zang
    • Yong-Feng Li
    • Xin-Yuan Guan
    • Jian-Hua Fu
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijo_00000497
  • Pages: 265-274
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To gain insights into the mechanisms of drug resistance in esophageal squamous cell carcinoma (ESCC), we employed proteomic techniques to study the global protein change of the multi-drug resistant ESCC cell line EC109/CDDP, which was established in our previous work, in comparison with its parental drug sensitive cell line EC109. By two-dimensional electrophoresis and mass spectrometry, we successfully identified 44 proteins with altered expression levels. These proteins are involved in endoplasmic reticulum stress response, metabolic process, DNA replication and repair, nucleotide binding, calcium binding, and cytoskeletal proteins. Among them, the differential expression levels of thioredoxin domain-containing protein 4 precursor and cystathionine γ-lyase were further validated by Western blot and RT-PCR. Our present results lay foundation for future in-depth work on molecular mechanism of ESCC drug resistance, and aid in the identification and use of novel markers in clinical practice.

Related Articles

Journal Cover

January 2010
Volume 36 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Wen, J., Zheng, B., Hu, Y., Zhang, X., Yang, H., Li, Y. ... Fu, J. (2010). Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP. International Journal of Oncology, 36, 265-274. https://doi.org/10.3892/ijo_00000497
MLA
Wen, J., Zheng, B., Hu, Y., Zhang, X., Yang, H., Li, Y., Zhang, C., Luo, K., Zang, X., Li, Y., Guan, X., Fu, J."Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP". International Journal of Oncology 36.1 (2010): 265-274.
Chicago
Wen, J., Zheng, B., Hu, Y., Zhang, X., Yang, H., Li, Y., Zhang, C., Luo, K., Zang, X., Li, Y., Guan, X., Fu, J."Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP". International Journal of Oncology 36, no. 1 (2010): 265-274. https://doi.org/10.3892/ijo_00000497