Frequent aberrant methylation of the imprinted IGF2/H19 locus and LINE1 hypomethylation in ovarian carcinoma

  • Authors:
    • Reinhard H. Dammann
    • Sebastian Kirsch
    • Undraga Schagdarsurengin
    • Temuujin Dansranjavin
    • Elise Gradhand
    • Wolfgang D. Schmitt
    • Steffen Hauptmann
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijo_00000488
  • Pages: 171-179
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Abstract

Epigenetic alteration of tumor-related genes through changes of DNA methylation is a hallmark for carcinogenesis and aberrant DNA methylation modulates the activity of tumor suppressor genes, imprinted genes and repetitive elements. In ovarian carcinoma, frequent loss of imprinting or aberrant methylation of repetitive elements were reported, however, combined analysis were not performed. We analyzed the aberrant methylation of a differentially methylated region (DMR0) and a CTCF binding site of the IGF2-H19 locus and methylation of LINE1 and Satellite 2 in 22 primary ovarian carcinomas (OC) and controls by a quantitative bisulfite restriction analysis (QUBRA). In 91% of OC, a significant hypomethylation of DMR0 was found compared to controls (p<0.05). In 77% of OC, a hypermethylation of a CTCF binding site was found (p<0.05). A combined hypomethylation of DMR0 and hypermethylation of the CTCF binding was observed in 73% of OC. Hypomethylation of LINE1 and Satellite 2 was detected in 100 and 23% of OC, respectively. In summary, we found frequent combined aberrant methylation of the IGF2-H19 locus and LINE1 in the vast majority of OC, suggesting that these changes are important events in tumorigenesis.

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January 2010
Volume 36 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Dammann, R.H., Kirsch, S., Schagdarsurengin, U., Dansranjavin, T., Gradhand, E., Schmitt, W.D., & Hauptmann, S. (2010). Frequent aberrant methylation of the imprinted IGF2/H19 locus and LINE1 hypomethylation in ovarian carcinoma. International Journal of Oncology, 36, 171-179. https://doi.org/10.3892/ijo_00000488
MLA
Dammann, R. H., Kirsch, S., Schagdarsurengin, U., Dansranjavin, T., Gradhand, E., Schmitt, W. D., Hauptmann, S."Frequent aberrant methylation of the imprinted IGF2/H19 locus and LINE1 hypomethylation in ovarian carcinoma". International Journal of Oncology 36.1 (2010): 171-179.
Chicago
Dammann, R. H., Kirsch, S., Schagdarsurengin, U., Dansranjavin, T., Gradhand, E., Schmitt, W. D., Hauptmann, S."Frequent aberrant methylation of the imprinted IGF2/H19 locus and LINE1 hypomethylation in ovarian carcinoma". International Journal of Oncology 36, no. 1 (2010): 171-179. https://doi.org/10.3892/ijo_00000488