Exosomes derived from IL-12-anchored renal cancer cells increase induction of specific antitumor response in vitro: A novel vaccine for renal cell carcinoma

  • Authors:
    • Yao Zhang
    • Chun-Li Luo
    • Bai-Cheng He
    • Jia-Mo Zhang
    • Gang Cheng
    • Xiao-Hou Wu
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijo_00000484
  • Pages: 133-140
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Abstract

Exosome-based immunotherapy for cancer holds promise, but needs improvements, especially for tumor-derived exosomes. We investigated, whether exosomes derived from IL-12-anchored human renal cancer cells could enhance their immunogenicity and increase induction of specific antitumor response. A mammalian co-expression plasmid of glycolipid-anchored-IL-12 (GPI-IL-12) was constructed by subcloning IL-12A chain gene (P35 subunit) and a fusion gene containing GPI-anchor signal sequence of human placental alkaline phosphatase-1 (hPLAP-1) and IL-12B chain gene (P40 subunit) in pBudCE4.1. Then exosomes were prepared from renal cancer cells modified to express GPI-IL-12. The results showed that exosomes derived from IL-12-anchored renal cancer cells expressed renal cell carcinoma-associated antigen G250 and GPI-IL-12. The incorporation of GPI-IL-12 onto exosomes (exosomes-GPI-IL-12, EXO/IL-12) significantly promotes proliferation of T cells, and subsequently increased the release of IFN-γ. Notably, stimulation with EXOs/IL-12 could efficiently induce antigen-specific cytotoxic T lymphocytes (CTLs), resulting in more significant cytotoxic effects in vitro. These results suggested that exosomes derived from IL-12-anchored renal cancer cells bore GPI-IL-12 and G250, which have tumor rejection antigen with enhanced immunogenicity and antitumor effects, representing a novel strategy of exosomes-based vaccine for renal cell carcinoma.

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January 2010
Volume 36 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Zhang, Y., Luo, C., He, B., Zhang, J., Cheng, G., & Wu, X. (2010). Exosomes derived from IL-12-anchored renal cancer cells increase induction of specific antitumor response in vitro: A novel vaccine for renal cell carcinoma. International Journal of Oncology, 36, 133-140. https://doi.org/10.3892/ijo_00000484
MLA
Zhang, Y., Luo, C., He, B., Zhang, J., Cheng, G., Wu, X."Exosomes derived from IL-12-anchored renal cancer cells increase induction of specific antitumor response in vitro: A novel vaccine for renal cell carcinoma". International Journal of Oncology 36.1 (2010): 133-140.
Chicago
Zhang, Y., Luo, C., He, B., Zhang, J., Cheng, G., Wu, X."Exosomes derived from IL-12-anchored renal cancer cells increase induction of specific antitumor response in vitro: A novel vaccine for renal cell carcinoma". International Journal of Oncology 36, no. 1 (2010): 133-140. https://doi.org/10.3892/ijo_00000484