Target chemotherapy of anti-CD147 antibody-labeled liposome encapsulated GSH-DXR conjugate on CD147 highly expressed carcinoma cells

  • Authors:
    • Hiroshi Matsudaira
    • Tadashi Asakura
    • Katsuhiko Aoki
    • Yasuyuki Searashi
    • Tomokazu Matsuura
    • Hisato Nakajima
    • Hisao Tajiri
    • Kiyoshi Ohkawa
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/ijo_00000477
  • Pages: 77-83
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Abstract

It was confirmed that CD147 (Emmprin) was expressed on the cell surface of carcinoma cells. For the purpose of studying the efficacy of a CD147-targeting agent on CD147-expressing carcinoma cells, we investigated the effect of a conjugate of glutathione-doxorubicin (GSH-DXR) encapsulated in an anti-CD147 antibody-labeled liposome (aCD147ab-liposome) in terms of specific accumulation and cytotoxicity in CD147-expressing human carcinoma cells. Expression of CD147 was not observed in many normal human tissues. However, slight expression of CD147 in kidney, prostate and breast tissues was observed. By contrast, high-level expression of CD147 in all carcinoma cells such as A431, PC3 and Ishikawa cell lines was confirmed by fluorescent microscopy and Western blot analysis. Specific accumulation of the aCD147ab-liposome in the above-described CD147-expressing cells was observed. GSH-DXR encapsulated in an aCD147ab-liposome expressed specific cytotoxicity against these carcinoma cells. These results suggested that target chemotherapy of GSH-DXR encapsulated in an aCD147ab-liposome on CD147-expressing carcinoma cells was effective.

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January 2010
Volume 36 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Matsudaira, H., Asakura, T., Aoki, K., Searashi, Y., Matsuura, T., Nakajima, H. ... Ohkawa, K. (2010). Target chemotherapy of anti-CD147 antibody-labeled liposome encapsulated GSH-DXR conjugate on CD147 highly expressed carcinoma cells. International Journal of Oncology, 36, 77-83. https://doi.org/10.3892/ijo_00000477
MLA
Matsudaira, H., Asakura, T., Aoki, K., Searashi, Y., Matsuura, T., Nakajima, H., Tajiri, H., Ohkawa, K."Target chemotherapy of anti-CD147 antibody-labeled liposome encapsulated GSH-DXR conjugate on CD147 highly expressed carcinoma cells". International Journal of Oncology 36.1 (2010): 77-83.
Chicago
Matsudaira, H., Asakura, T., Aoki, K., Searashi, Y., Matsuura, T., Nakajima, H., Tajiri, H., Ohkawa, K."Target chemotherapy of anti-CD147 antibody-labeled liposome encapsulated GSH-DXR conjugate on CD147 highly expressed carcinoma cells". International Journal of Oncology 36, no. 1 (2010): 77-83. https://doi.org/10.3892/ijo_00000477