Rassf3 is responsible in part for resistance to mammary tumor development in neu transgenic mice

  • Authors:
    • Isabelle C. Jacquemart
    • Alison E.B. Springs
    • Wen Y. Chen
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/ijo_00000177
  • Pages: 517-528
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Abstract

MMTV/neu transgenic mouse line is a well-documented model for studying HER2/neu-related breast cancer. Approximately 80% of these mice develop mammary tumors by 11 months of age, whereas a small percentage appears to have naturally acquired resistance to HER2/neu tumorigenesis. To identify factors responsible for tumor resistance in these transgenic mice, comparative genetic profiling was used to screen alterations in gene expression in the mammary gland. A novel gene, the RAS association domain (RalGDS/AF-6) family 3 (Rassf3), which belongs to a family of RAS effectors and tumor suppressor genes, was identified. Data indicated 1) that Rassf3 is overexpressed in mammary gland of tumor-resistant MMTV/neu mice compared to tumor-susceptible MMTV/neu littermates or non-transgenic mice, and 2) Rassf3 is significantly up-regulated in neu-specific mouse mammary tumors compared to adjacent normal tissues. In vitro overexpression of RASSF3 inhibited cell proliferation in HER2/neu positive human and mouse breast cancer cell lines, possibly through induction of apoptosis. A novel MMTV/Rassf3-neu bi-transgenic mouse line, overexpressing Rassf3 and neu genes in mammary glands, was established. Mammary tumor incidence in bi-transgenic mice was delayed compared to their MMTV/neu+/− littermates. These data suggest that Rassf3 may influence mammary tumor incidence in MMTV/neu transgenic mice.

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February 2009
Volume 34 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Jacquemart, I.C., Springs, A.E., & Chen, W.Y. (2009). Rassf3 is responsible in part for resistance to mammary tumor development in neu transgenic mice. International Journal of Oncology, 34, 517-528. https://doi.org/10.3892/ijo_00000177
MLA
Jacquemart, I. C., Springs, A. E., Chen, W. Y."Rassf3 is responsible in part for resistance to mammary tumor development in neu transgenic mice". International Journal of Oncology 34.2 (2009): 517-528.
Chicago
Jacquemart, I. C., Springs, A. E., Chen, W. Y."Rassf3 is responsible in part for resistance to mammary tumor development in neu transgenic mice". International Journal of Oncology 34, no. 2 (2009): 517-528. https://doi.org/10.3892/ijo_00000177