Open Access

Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes

  • Authors:
    • Ayguen Sahin
    • Chantal Vercamer
    • Annette Kaminski
    • Tanja Fuchs
    • Alexandra Florin
    • Jens Claus Hahne
    • Virginie Mattot
    • Albin Pourtier-Manzanedo
    • Torsten Pietsch
    • Véronique Fafeur
    • Nicolas Wernert
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/ijo_00000161
  • Pages: 377-389
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Abstract

We previously reported that the inactivation of the Ets 1 transcription factor by a specific decoy strategy reduces rat C6 glioma cell proliferation and mmp-9 expression. In the present study, we analysed the effects of the dominant-negative form of Ets 1 (Ets-DB) on rat C6 glioma cell proliferation, migration, invasion, in vivo tumor growth on the chicken chorioallantoic membrane (CAM) and mmp-9 expression. In addition, we examined differences in gene expression between Ets-DB expressing and control cells using suppression subtractive hybridization (SSH). We found that retrovirus mediated expression of Ets-DB inhibited cellular proliferation, migration, invasion, mmp-9 expression, cellular growth in soft agar, and in vivo growth in the chicken chorioallantoic membrane assay. SSH analysis revealed expression of different genes in Ets-DB expressing cells involved in basic cellular processes. Each of these genes contained binding sites for different Ets-factors within their promoters. Finally, we found that, in addition to Ets 1, Elk-1, Elf-1, Fli-1 and Etv-1 are further Ets family members expressed in rat C6 glioma cells. Our results indicate that Ets transcription factors play important roles for basic properties of rat C6 glioma cells. Targeting of these factors might therefore become a useful experimental tool for therapeutic strategies against malignant gliomas.

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February 2009
Volume 34 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

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Copy and paste a formatted citation
APA
Sahin, A., Vercamer, C., Kaminski, A., Fuchs, T., Florin, A., Hahne, J.C. ... Wernert, N. (2009). Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes. International Journal of Oncology, 34, 377-389. https://doi.org/10.3892/ijo_00000161
MLA
Sahin, A., Vercamer, C., Kaminski, A., Fuchs, T., Florin, A., Hahne, J. C., Mattot, V., Pourtier-Manzanedo, A., Pietsch, T., Fafeur, V., Wernert, N."Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes". International Journal of Oncology 34.2 (2009): 377-389.
Chicago
Sahin, A., Vercamer, C., Kaminski, A., Fuchs, T., Florin, A., Hahne, J. C., Mattot, V., Pourtier-Manzanedo, A., Pietsch, T., Fafeur, V., Wernert, N."Dominant-negative inhibition of Ets 1 suppresses tumor growth, invasion and migration in rat C6 glioma cells and reveals differentially expressed Ets 1 target genes". International Journal of Oncology 34, no. 2 (2009): 377-389. https://doi.org/10.3892/ijo_00000161