Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells

  • Authors:
    • Kosuke Narita
    • Takenori Fujii
    • Toshiyuki Ishiwata
    • Tetsushi Yamamoto
    • Yoko Kawamoto
    • Kiyoko Kawahara
    • Nando Nakazawa
    • Zenya Naito
  • View Affiliations

  • Published online on: February 1, 2009     https://doi.org/10.3892/ijo_00000158
  • Pages: 355-360
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Keratinocyte growth factor (KGF), which is also called fibroblast growth factor (FGF)-7, belongs to the FGF family. KGF is not commonly produced by human cancer cells, but the KGF receptor (KGFR) is expressed in most cancer cells and particularly highly expressed in well-differentiated types of cancer. Recently, it has been reported that vascular endothelial growth factor (VEGF)-A expression is induced by KGF in pancreatic cancer cells. VEGF-A is produced by some cancer cells and plays important roles in the angiogenesis and metastasis of cancer cells including those in the colorectum. In this study, we examined whether recombinant human KGF (rhKGF) induces major angiogenic growth factors including VEGF-A, FGF-2 and hepatocyte growth factor (HGF) in human colorectal cancer cells (HCT-15), which express a high level of KGFR, but a low or negligible level of KGF. rhKGF significantly increased the VEGF-A expression level in a serum-free medium of HCT-15 cells, but FGF-2 and HGF expression levels were too low to detect. Furthermore, the expression levels of the angiogenic growth factors were evaluated in KGF-transfected HCT-15 cells, which were induced to stably overexpress KGF by KGF gene transfection and mock-transfected cells (Mock). KGF and VEGF-A expression levels in the cells and the protein concentrations in serum-free medium were significantly higher in KGF-transfected HCT-15 cells than in Mock cells. In contrast, the FGF-2 and HGF mRNA expression levels were not significantly different between KGF-transfected HCT-15 cells and Mock cells and the protein concentrations in serum-free medium of the cells were below the detection level. These findings suggest that administration of rhKGF and over-expression of endogenous KGF genes in colorectal cancer cells increase VEGF-A production and may relate to angiogenesis in cancer.

Related Articles

Journal Cover

February 2009
Volume 34 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Narita, K., Fujii, T., Ishiwata, T., Yamamoto, T., Kawamoto, Y., Kawahara, K. ... Naito, Z. (2009). Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells. International Journal of Oncology, 34, 355-360. https://doi.org/10.3892/ijo_00000158
MLA
Narita, K., Fujii, T., Ishiwata, T., Yamamoto, T., Kawamoto, Y., Kawahara, K., Nakazawa, N., Naito, Z."Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells". International Journal of Oncology 34.2 (2009): 355-360.
Chicago
Narita, K., Fujii, T., Ishiwata, T., Yamamoto, T., Kawamoto, Y., Kawahara, K., Nakazawa, N., Naito, Z."Keratinocyte growth factor induces vascular endothelial growth factor-A expression in colorectal cancer cells". International Journal of Oncology 34, no. 2 (2009): 355-360. https://doi.org/10.3892/ijo_00000158