In vivo monitoring of splenocytes in NMRI nu/nu- and nu/+,- mice during Lewis lung tumor progression or regression

  • Authors:
    • C Lersch
    • M Schunke
    • C Hammer
  • View Affiliations

  • Published online on: June 1, 1996     https://doi.org/10.3892/ijo.8.6.1213
  • Pages: 1213-1216
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Abstract

Splenocytes of T-cell deficient NMRI nu/nu- mice (n=15) and of inbred immunocompetent NMRI nu/+,littermates (n=18) significantly (p<0.05) increased during Lewis lung (LL) tumor growth and eventually decreased. Half of the heterozygous NMRI nu/+,- mice rejected the tumors (LL-regressors). Zymosan-induced and lucigenin-amplified chemiluminescence (CL) of splenic polymorphonuclear leukocytes (PMN) and -precursors significantly (p<0.05) increased in NMRI nu/+,- mice with progressing tumors (LL-progressors) on the 3rd and in LL-regressors and nude mice on the 7th day after tumor inoculation. It subsequently decreased although numbers of PMN/- precursors further multiplied predominantly in the LL-progressors. Another peak of CL was found in those animals after the 20th day. Splenic lymphocytes significantly (p<0.05) increased in all mice from day 5 forward and dropped to original numbers between the 20th and 25th days. Maximal lymphocyte counts were significantly (p<0.05) lower in nude mice as compared to the NMRI nu/+,- littermates. The splenic T-/B-ratio significantly (p<0.005) correlated in LL-regressors and did not in LL-progressors. This correlation could be a suitable marker for tumor progression in NMRI nu/+ mice bearing LL-tumors.

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June 1996
Volume 8 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Lersch, C., Schunke, M., & Hammer, C. (1996). In vivo monitoring of splenocytes in NMRI nu/nu- and nu/+,- mice during Lewis lung tumor progression or regression. International Journal of Oncology, 8, 1213-1216. https://doi.org/10.3892/ijo.8.6.1213
MLA
Lersch, C., Schunke, M., Hammer, C."In vivo monitoring of splenocytes in NMRI nu/nu- and nu/+,- mice during Lewis lung tumor progression or regression". International Journal of Oncology 8.6 (1996): 1213-1216.
Chicago
Lersch, C., Schunke, M., Hammer, C."In vivo monitoring of splenocytes in NMRI nu/nu- and nu/+,- mice during Lewis lung tumor progression or regression". International Journal of Oncology 8, no. 6 (1996): 1213-1216. https://doi.org/10.3892/ijo.8.6.1213