Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas

  • Authors:
    • A Noutsou
    • M Koffa
    • M Ergazaki
    • N Siafakas
    • D Spandidos
  • View Affiliations

  • Published online on: June 1, 1996     https://doi.org/10.3892/ijo.8.6.1089
  • Pages: 1089-1093
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Abstract

The purpose of our study was to assess the prevalence and prognostic significance of HPV infection as well as K-ras codon 12 point mutations in lung cancer. Patients diagnosed with lung carcinoma between 1988 and 1992 (N=99) were selected. HPV detection and typing was performed by PCR from paraffin-embedded tissues, while mutations in codon 12 of K-ras gene were detected using the restriction fragment length polymorphism (RFLP) analysis. The prevalence of HPV infection was 15%, while K-ras codon 12 point mutations were found in 18% of the specimens examined. In 50% of the HPV-positive cases, K-ras gene mutation coexisted. HPV 18 was the most frequent type. No correlation was found between K-ras mutation and HPV infection with sex, age and clinical outcome of the patient, or the histological type and the differentiation grade of the tumor. An association was found between K-ms codon 12 point mutations and the stage of the tumor, occurring more frequently at stage III (p=0.037). Infection with potentially oncogenic HPV types could co-operate with K-ras gene activation in the progression of the disease, since K-ras activation by point mutations seems to be a late event in lung carcinogenesis.

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June 1996
Volume 8 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Noutsou, A., Koffa, M., Ergazaki, M., Siafakas, N., & Spandidos, D. (1996). Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas. International Journal of Oncology, 8, 1089-1093. https://doi.org/10.3892/ijo.8.6.1089
MLA
Noutsou, A., Koffa, M., Ergazaki, M., Siafakas, N., Spandidos, D."Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas". International Journal of Oncology 8.6 (1996): 1089-1093.
Chicago
Noutsou, A., Koffa, M., Ergazaki, M., Siafakas, N., Spandidos, D."Detection of human papilloma virus (HPV) and K-ras mutations in human lung carcinomas". International Journal of Oncology 8, no. 6 (1996): 1089-1093. https://doi.org/10.3892/ijo.8.6.1089