Focal distribution of p16/CDKN2 gene mutations within individual prostate carcinomas

  • Authors:
    • N Konishi
    • Y Hiasa
    • M Tao
    • H Matsuda
    • M Nakamura
    • K Yane
    • Y Kitahori
  • View Affiliations

  • Published online on: March 1, 1996     https://doi.org/10.3892/ijo.8.3.549
  • Pages: 549-554
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Abstract

To examine the potential role of pl6/CDKN2 gene mutations in prostate tumorigenesis, focal areas within individual tumors were investigated. Eleven cases of histologically heterogeneous prostate carcinomas obtained by radical prostatectomy were subjected to analysis of p16/CDKN2 gene mutations. DNA was extracted from 5 to 10 separate areas of each tumor with different growth or histological patterns. Exons 1 through 3 of the p16/CDKN2 gene were amplified using the polymerase chain reaction (PCR) and screened for homozygous deletions and mutations of this gene by single-strand conformation polymorphism (SSCP) analysis. No homozygous deletions were observed in any of the prostate carcinomas, but two of the eleven tumors demonstrated mutations in exon 2 of p16/CDKN2 gene. Missense mutations were detected in only one and two foci, respectively, out of six ana ten selected tumor areas. The present results suggest that p16/CDKN2 gene mutations, although they occur at a low incidence, are involved in prostate tumorigenesis, indicating a mutational heterogeneity in addition to morphological heterogeneity.

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March 1996
Volume 8 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Konishi, N., Hiasa, Y., Tao, M., Matsuda, H., Nakamura, M., Yane, K., & Kitahori, Y. (1996). Focal distribution of p16/CDKN2 gene mutations within individual prostate carcinomas. International Journal of Oncology, 8, 549-554. https://doi.org/10.3892/ijo.8.3.549
MLA
Konishi, N., Hiasa, Y., Tao, M., Matsuda, H., Nakamura, M., Yane, K., Kitahori, Y."Focal distribution of p16/CDKN2 gene mutations within individual prostate carcinomas". International Journal of Oncology 8.3 (1996): 549-554.
Chicago
Konishi, N., Hiasa, Y., Tao, M., Matsuda, H., Nakamura, M., Yane, K., Kitahori, Y."Focal distribution of p16/CDKN2 gene mutations within individual prostate carcinomas". International Journal of Oncology 8, no. 3 (1996): 549-554. https://doi.org/10.3892/ijo.8.3.549