Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I)

  • Authors:
    • R Pfragner
    • G Wirnsberger
    • B Niederle
    • A Behmel
    • I Rinner
    • A Mandl
    • F Wawrina
    • J Luo
    • D Adamiker
    • H Hoger
    • E Ingolic
    • K Schauenstein
  • View Affiliations

  • Published online on: March 1, 1996     https://doi.org/10.3892/ijo.8.3.513
  • Pages: 513-520
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Abstract

A new continuous cell line from a human malignant carcinoid of the small intestine (KRJ-I) was established. The cells showed morphological and immunocytochemical features of the tumor of origin and expressed estrogen receptors. The cells are growing as a suspension, forming multicellular aggregates and spheroids. Electron microscopy confirmed the presence of neuroendocrine granules. Dose-dependent growth inhibition was observed after incubation with 5-azacytidine. Cytogenetic analyses of the tumor of origin, the cell line KRJ-I and a liver metastasis KRJ-II revealed clonal tetraploidy and clonal loss of the Y-chromosome and chromosome 19.

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March 1996
Volume 8 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Pfragner, R., Wirnsberger, G., Niederle, B., Behmel, A., Rinner, I., Mandl, A. ... Schauenstein, K. (1996). Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I). International Journal of Oncology, 8, 513-520. https://doi.org/10.3892/ijo.8.3.513
MLA
Pfragner, R., Wirnsberger, G., Niederle, B., Behmel, A., Rinner, I., Mandl, A., Wawrina, F., Luo, J., Adamiker, D., Hoger, H., Ingolic, E., Schauenstein, K."Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I)". International Journal of Oncology 8.3 (1996): 513-520.
Chicago
Pfragner, R., Wirnsberger, G., Niederle, B., Behmel, A., Rinner, I., Mandl, A., Wawrina, F., Luo, J., Adamiker, D., Hoger, H., Ingolic, E., Schauenstein, K."Establishment of a continuous cell line from a human carcinoid of the small intestine (KRJ-I)". International Journal of Oncology 8, no. 3 (1996): 513-520. https://doi.org/10.3892/ijo.8.3.513