Autocrine transforming growth factor-beta (TGF beta) modulates the 120 kD and 105 kD retinoblastoma (RB) protein species in CBS colon carcinoma cells as revealed by an anti-sense TGF beta 1 expression vector
- C Dobre
- L Humphrey
- M Brattain
Affiliations: MED COLL OHIO,DEPT BIOCHEM & MOLEC BIOL,TOLEDO,OH 43614.
- Published online on: March 1, 1996 https://doi.org/10.3892/ijo.8.3.469
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Specific RE protein species modulated by autocrine TGF beta are unknown. CBS NEO (with autocrine TGF beta) and antisense TGF beta 1 transfected CBS RSVAS cells were compared for mitogenesis and RE species modulation. Significantly, high MW (120, 130 kD) were abundant relative to low MW (116, 110, 105 kD combined) RE species. CBS NEO required growth factors (GF) for maximal DNA synthesis (14-fold). 120 kD and 105 kD were significantly modulated in CBS NEO-GF. Nutrients (N) were an sufficient to maximally stimulate CBS RSVAS (8-fold). 130 kD and 116-110 low MW species were moderately induced by both N (CBS RSVAS-N) and GF (CBS NEO-GF), independent of autocrine TGF beta. In conclusion, autocrine TGF beta (CBS) has a role in modulation of 120 kD and 105 kD RE species which may play a role in the inhibitory action of autocrine TGF beta pathway.