G-CSF accelerates the cell-cycling of hematopoietic progenitor cells and abrogates the deceleration by TNF alpha

  • Authors:
    • N Mahmud
    • N Katayama
    • R Itoh
    • K Ohishi
    • M Masuya
    • N Minami
    • H Shiku
  • View Affiliations

  • Published online on: March 1, 1996     https://doi.org/10.3892/ijo.8.3.453
  • Pages: 453-459
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Abstract

We examined the effects of granulocyte colony-stimulating factor (G-CSF) on cell-cycling of hematopoietic progenitors in serum-free methylcellulose clonal cultures. Serial observations of the cultures showed hastening of growth of colonies by G-CSF, as determined by evaluating the time for individual colonies of 20 cells to reach 40 cells. G-CSF did not affect the incidence of proliferating cells in each developing colony. Cell-cycle analysis revealed that addition of G-CSF to cultures led to a decrease in the percentage of cells in the G1 phase of the cell-cycle, thereby indicating that G-CSF can modulate the cell-cycle of hematopoietic progenitors mainly by shortening the period of the G1 phase. Tumor necrosis factor alpha (TNF alpha) exerted opposite effects on cell-cycling of hematopoietic progenitors to those seen with G-CSF. G-CSF abolished the inhibitory effects of TNF alpha on the cell-cycling of hematopoietic progenitors. These observations indicate positive and negative regulatory roles of C-CSF and TNF alpha, respectively, and their interactions in the regulation of cell-cycling of hematopoietic progenitors.

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March 1996
Volume 8 Issue 3

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
Mahmud, N., Katayama, N., Itoh, R., Ohishi, K., Masuya, M., Minami, N., & Shiku, H. (1996). G-CSF accelerates the cell-cycling of hematopoietic progenitor cells and abrogates the deceleration by TNF alpha. International Journal of Oncology, 8, 453-459. https://doi.org/10.3892/ijo.8.3.453
MLA
Mahmud, N., Katayama, N., Itoh, R., Ohishi, K., Masuya, M., Minami, N., Shiku, H."G-CSF accelerates the cell-cycling of hematopoietic progenitor cells and abrogates the deceleration by TNF alpha". International Journal of Oncology 8.3 (1996): 453-459.
Chicago
Mahmud, N., Katayama, N., Itoh, R., Ohishi, K., Masuya, M., Minami, N., Shiku, H."G-CSF accelerates the cell-cycling of hematopoietic progenitor cells and abrogates the deceleration by TNF alpha". International Journal of Oncology 8, no. 3 (1996): 453-459. https://doi.org/10.3892/ijo.8.3.453