CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA

  • Authors:
    • JK SAGOO
    • C IAONNOU
    • NRA BEELEY
    • C SUTTON
    • CI DEMATTEIS
    • SJB TENDLER
  • View Affiliations

  • Published online on: December 1, 1995     https://doi.org/10.3892/ijo.7.6.1437
  • Pages: 1437-1443
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Abstract

We report the characterization of a 14 kDa degradation fragment from recombinant human tumour necrosis factor-alpha (TNF alpha) by N-terminal sequencing and mass spectrometry. A single site between the dibasic residues Arg(31)-Arg(32) of the mature recombinant 17 kDa protein has been identified as the target site that generates the 14 kDa fragment. The observation that a maximum of 33% degradation occurs suggests that only one monomer per TNF trimer is cleaved. E. coli proteases specific for dibasic residues are thought to be responsible for this cleavage. A strategy has been developed which completely inhibits proteolysis. This strategy has been used to reduce the 14 kDa degradation fragment obtained from approximately 33% of the total purified protein to zero.

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December 1995
Volume 7 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

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APA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DEMATTEIS, C., & TENDLER, S. (1995). CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA. International Journal of Oncology, 7, 1437-1443. https://doi.org/10.3892/ijo.7.6.1437
MLA
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DEMATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7.6 (1995): 1437-1443.
Chicago
SAGOO, J., IAONNOU, C., BEELEY, N., SUTTON, C., DEMATTEIS, C., TENDLER, S."CHARACTERIZATION OF THE 14 KDA FRAGMENT OF HUMAN TUMOR-NECROSIS-FACTOR-ALPHA". International Journal of Oncology 7, no. 6 (1995): 1437-1443. https://doi.org/10.3892/ijo.7.6.1437