EXPRESSION OF MUCINOUS OVARIAN-CANCER ANTIGEN IN HYBRID-CELLS DERIVED BY FUSING A MALIGNANTLY TRANSFORMED BLOOM-SYNDROME CELL-LINE (BS-SHI-4M OVC-MU) AND MOUSE L-CELL-LINE
- Y SHIRAISHI
- T TAGUCHI
- H SEGUCHI
Affiliations: KOCHI MED SCH,DEPT ANAT 2,NANKOKU,KOCHI 783,JAPAN.
- Published online on: December 1, 1995 https://doi.org/10.3892/ijo.7.6.1275
Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
This article is mentioned in:
The expression of mucinous ovarian cancer (OVC) antigen by hybrid cell lines derived by fusing a malignantly transformed Bloom syndrome (BS) cell line (BS-SHI-4M OVC-MU) and mouse L cells has been studied. Cell surfaces of 16 hybrids which grew 12-40 days post-fusion have been analysed by using a number of sera from mucinous OVC patients and indirect membrane immunofluorescence (IF). Three hybrids which appeared at a late stage (30-40 days in 24-well culture plates) were clearly positive in almost 100% of the hybrid cell population, while all of those which appeared earlier (in 12 days) were completely negative. Cytogenetic analysis showed that these three hybrids with positive mucinous OVC antigen contained human chromosome 22 or 22q-, though no metaphases from antigen negative population had 22 or 22q-. Therefore, we assign the gene for mucinous OVC antigen to the chromosome 22. Identification of chromosome 22 in hybrid cells was confirmed by the fluorescence in situ hybridization (FISH) analysis using chromosome 22 painting probe.